Molecular mechanism of temperature sensing by the circadian clock of Neurospora crassa

被引:102
作者
Diernfellner, ACR
Schafmeier, T
Merrow, MW
Brunner, M [1 ]
机构
[1] Heidelberg Univ, Biochem Ctr, BZH, D-69120 Heidelberg, Germany
[2] Univ Groningen, Dept Behav Biol, NL-9750 AA Haren, Netherlands
关键词
circadian clock; temperature compensation; alternative splicing; translational control;
D O I
10.1101/gad.345905
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Expression levels and ratios of the long (1) and short (s) isoforms of the Neurospora circadian clock protein FREQUENCY (FRQ) are crucial for temperature compensation of circadian rhythms. We show that the ratio of 1-FRQ versus s-FRQ is regulated by thermosensitive splicing of intron 6 of frq, a process removing the translation initiation site of 1-FRQ. Thermosensitivity is due to inefficient recognition of nonconsensus splice sites at elevated temperature. The temperature-dependent accumulation of FRQ relative to bulk protein is controlled at the level of translation. The 5'-UTR of frq RNA contains six upstream open reading frames (uORFs) that are in nonconsensus context for translation initiation. Thermosensitive trapping of scanning ribosomes at the uORFs leads to reduced translation of the main ORF and allows adjustment of FRQ levels according to ambient temperature.
引用
收藏
页码:1968 / 1973
页数:6
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