Penetratin-induced aggregation and subsequent dissociation of negatively charge phospholipid vesicles

被引：76

作者：

Persson, D ^{[1
]}

Thorén, PEG ^{[1
]}

Nordén, B ^{[1
]}

机构：

[1] Chalmers Univ Technol, Dept Phys Chem, SE-41296 Gothenburg, Sweden

来源：

FEBS LETTERS | 2001年 / 505卷 / 02期

关键词：

Antennapedia homeodomain; penetratin; vesicle aggregation; circular dichroism; artifact; turbidity;

D O I：

10.1016/S0014-5793(01)02843-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The interaction of the cellular delivery vector penetratin with a model system consisting of negatively charged phospholipid vesicles has been studied. Above a certain peptide to lipid molar ratio, the cationic oligopeptide induces vesicle aggregation. Interestingly, the aggregation is followed by spontaneous disaggregation, which may be related to membrane translocation of the peptide. Circular dichroism (CD) measurements indicate a conformational transition, from alpha -helix to antiparallel beta -pleated sheet, which is simultaneous with the aggregation process. The potential influence of spectroscopic artifacts on CD data due to the drastically increased turbidity during aggregation is discussed. (C) 2001 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.

引用

页码：307 / 312

页数：6

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