Proteomic analysis using protein chips to detect biomarkers in cervical and amniotic fluid in women with intra-amniotic inflammation

被引：95

作者：

Rüetschi, U

Rosén, Å

Karlsson, G

Zetterberg, H

Rymo, L

Hagberg, H

Jacobsson, B ^{[1
]}

机构：

[1] Sahlgrens Univ Hosp, Inst Hlth Women & Children, Perinatal Ctr, Dept Obstet & Gynecol, SE-41685 Gothenburg, Sweden

[2] Sahlgrens Univ Hosp, Dept Clin Chem & Transfus Med, SE-41685 Gothenburg, Sweden

[3] Univ Aarhus, NANEA, Dept Epidemiol & Social Med, Aarhus, Denmark

来源：

JOURNAL OF PROTEOME RESEARCH | 2005年 / 4卷 / 06期

关键词：

proteomics; surface-enhanced laser desorption ionization time-of-flight mass spectrometry; SELDI-TOF-MS; preterm birth; intra-amniotic inflammation; preterm labor; preterm prelabor rupture of membranes;

D O I：

10.1021/pr050139e

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Intra-amniotic inflammation (IAI) may cause preterm birth with poor neonatal out-come. To identify novel biomarkers for IAI, we analyzed amniotic and cervical fluid samples from 27 patients with signs of threatening preterm birth with or without IAI by surface-enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS). Seventeen proteins were significantly overexpressed in amniotic fluid from IAI cases and more often in women with preterm labor than those with rupture of membranes. Five of these were identified as human neutrophil protein 1-3, calgranulin A and B.

引用

页码：2236 / 2242

页数：7

共 31 条

[1] Proteomic biomarker analysis of amniotic fluid for identification of intra-amniotic inflammation [J].

Buhimschi, IA ;

Christner, R ;

Buhimschi, CS .

BJOG-AN INTERNATIONAL JOURNAL OF OBSTETRICS AND GYNAECOLOGY, 2005, 112 (02) :173-181

[2]

Espinoza J, 2003, J Matern Fetal Neonatal Med, V13, P2, DOI 10.1080/jmf.13.1.2.21

[3] Defensin-rich granules of human neutrophils: characterization of secretory properties [J].

Faurschou, M ;

Sorensen, OE ;

Johnsen, AH ;

Askaa, J ;

Borregaard, N .

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 2002, 1591 (1-3) :29-35

[4] Role of tumor necrosis factor-α in the premature rupture of membranes and preterm labor pathways [J].

Fortunato, SJ ;

Menon, R ;

Lombardi, SJ .

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 2002, 187 (05) :1159-1162

[5] Medical progress - Prevention of premature birth [J].

Goldenberg, RL ;

Rouse, DJ .

NEW ENGLAND JOURNAL OF MEDICINE, 1998, 339 (05) :313-320

[6] The fetal inflammatory response syndrome [J].

Gomez, R ;

Romero, R ;

Ghezzi, F ;

Yoon, BH ;

Mazor, M ;

Berry, SM .

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 1998, 179 (01) :194-202

[7] Pathogenesis of preterm labor and preterm premature rupture of membranes associated with intraamniotic infection [J].

Gomez, R ;

Romero, R ;

Edwin, SS ;

David, C .

INFECTIOUS DISEASE CLINICS OF NORTH AMERICA, 1997, 11 (01) :135-+

[8] Neutrophil granule products: Can they identify subclinical chorioamnionitis in patients with preterm premature rupture of membranes? [J].

Grable, IA ;

Heine, RP .

AMERICAN JOURNAL OF OBSTETRICS AND GYNECOLOGY, 2003, 189 (03) :808-812

[9] Changing panorama of cerebral palsy in Sweden. VIII. Prevalence and origin in the birth year period 1991-94 [J].

Hagberg, B ;

Hagberg, G ;

Beckung, E ;

Uvebrant, P .

ACTA PAEDIATRICA, 2001, 90 (03) :271-277

[10] Amniotic fluid defensins: Potential markers of subclinical intrauterine infection [J].

Heine, RP ;

Wiesenfeld, H ;

Mortimer, L ;

Greig, PC .

CLINICAL INFECTIOUS DISEASES, 1998, 27 (03) :513-518

← 1 2 3 4 →