Brain-derived and glial cell line-derived neurotrophic factors protect a catecholaminergic cell line from dopamine-induced cell death

被引：33

作者：

Gong, L ^{[1
]}

Wyatt, RJ ^{[1
]}

Baker, I ^{[1
]}

Masserano, JM ^{[1
]}

机构：

[1] NIMH, Neuropsychiat Branch, Bethesda, MD 20892 USA

来源：

NEUROSCIENCE LETTERS | 1999年 / 263卷 / 2-3期

关键词：

dopamine; cell death; glutathione; superoxide dismutase; brain-derived neurotrophic factor; glial cell line-derived neurotrophic factor; CATH.a cells;

D O I：

10.1016/S0304-3940(99)00148-2

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Brain-derived neurotrophic factor (BDNF) promotes the survival of dopaminergic neurons in primary cultures and protects these neurons from the neurotoxic effects of 6-hydroxydopamine. The protective mechanism of BDNF on neurotoxicity was evaluated using CATH.a cells, a clonal catecholaminergic cell line derived from the central nervous system. Dopamine produced a dose-dependent cell death in CATH.a cells. Treatment of CATH.a cells with BDNF or glia cell line-derived neurotrophic factor (GDNF) reduced dopamine-induced cell death by approximately 60-70%. Nerve growth factor, basic fibroblast growth factor, neurotrophin-4/5 and insulin had no protective effect on dopamine-induced cell death. Dopamine decreased the activity of superoxide dismutase and the levels of glutathione in the CATH.a cells and these decreases were reversed by BDNF. In addition, BDNF treatment alone increased superoxide dismutase activity by 108%, These results suggest that BDNF may safeguard CATH.a cells from dopamine-induced cell death by maintaining or enhancing components of the cell, which protect from oxidative stress. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：153 / 156

页数：4

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