Gold nano-particles (AuNPs) carrying anti-EBV-miR-BART7-3p inhibit growth of EBV-positive nasopharyngeal carcinoma

被引：56

作者：

Cai, Longmei ^{[1
]}

Li, Jinbang ^{[1
]}

Zhang, Xiaona ^{[1
,4
]}

Lu, Yaoyong ^{[1
,5
]}

Wang, Jianguo ^{[1
]}

Lyu, Xiaoming ^{[1
,3
]}

Chen, Yuxiang ^{[1
]}

Liu, Jinkun ^{[2
]}

Cai, Hongbing ^{[2
]}

Wang, Ying ^{[1
]}

Li, Xin ^{[1
]}

机构：

[1] Southern Med Univ, Canc Res Inst, Guangzhou 510515, Guangdong, Peoples R China

[2] Southern Med Univ, Sch Chinese Tradit Med, Guangzhou 510515, Guangdong, Peoples R China

[3] Southern Med Univ, Affiliated Hosp 3, Cent Med Lab, Guangzhou 510515, Guangdong, Peoples R China

[4] Sun Yat Sen Univ, Affiliated Hosp 6, Guangzhou 510655, Guangdong, Peoples R China

[5] Gaozhou Peoples Hosp, Dept Radiat Oncol, Gaozhou 525200, Peoples R China

来源：

ONCOTARGET | 2015年 / 6卷 / 10期

基金：

中国国家自然科学基金; 高等学校博士学科点专项科研基金;

关键词：

Nasopharyngeal carcinoma; EBV-miR-BART7; tumorigenesis; therapeutic experiment; CELL-PROLIFERATION; TUMOR-SUPPRESSOR; DOWN-REGULATION; C-MYC; CANCER; MICRORNA; TARGETS; INACTIVATION; PROMOTES; PATHWAY;

D O I：

10.18632/oncotarget.3046

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Epstein-Barr virus (EBV) infection is a major etiological factor for nasopharyngeal carcinoma (NPC). Several EBV-encoded BART miRNAs have been associated with viral latency, immune escape, cell survival, cell proliferation and apoptosis. Here, we report that EBV-miR-BART7-3p, an EBV-encoded BART miRNA highly expressed in NPC, was correlated with cell-cycle progression in vitro and increased tumor formation in vivo. This viral miRNA stimulated the PTEN/PI3K/Akt pathway and induced c-Myc and c-Jun. Knockdown of PTEN mimicked EBV-miR-BART7-3p-induced tumorigenic phenotype. Based on these results, we conducted a therapeutic experiment by using gold nano-particles (AuNPs) carrying anti-EBV-miR-BART7-3p. Silencing of EBV-miRBART7-3p reduced tumor growth in animal model. We conclude that EBV-miR-BART7-3p favors carcinogenesis, representing a potential target for miRNA-based therapy.

引用

页码：7838 / 7850

页数：13

共 62 条

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