Gold nano-particles (AuNPs) carrying anti-EBV-miR-BART7-3p inhibit growth of EBV-positive nasopharyngeal carcinoma

被引:56
作者
Cai, Longmei [1 ]
Li, Jinbang [1 ]
Zhang, Xiaona [1 ,4 ]
Lu, Yaoyong [1 ,5 ]
Wang, Jianguo [1 ]
Lyu, Xiaoming [1 ,3 ]
Chen, Yuxiang [1 ]
Liu, Jinkun [2 ]
Cai, Hongbing [2 ]
Wang, Ying [1 ]
Li, Xin [1 ]
机构
[1] Southern Med Univ, Canc Res Inst, Guangzhou 510515, Guangdong, Peoples R China
[2] Southern Med Univ, Sch Chinese Tradit Med, Guangzhou 510515, Guangdong, Peoples R China
[3] Southern Med Univ, Affiliated Hosp 3, Cent Med Lab, Guangzhou 510515, Guangdong, Peoples R China
[4] Sun Yat Sen Univ, Affiliated Hosp 6, Guangzhou 510655, Guangdong, Peoples R China
[5] Gaozhou Peoples Hosp, Dept Radiat Oncol, Gaozhou 525200, Peoples R China
基金
中国国家自然科学基金; 高等学校博士学科点专项科研基金;
关键词
Nasopharyngeal carcinoma; EBV-miR-BART7; tumorigenesis; therapeutic experiment; CELL-PROLIFERATION; TUMOR-SUPPRESSOR; DOWN-REGULATION; C-MYC; CANCER; MICRORNA; TARGETS; INACTIVATION; PROMOTES; PATHWAY;
D O I
10.18632/oncotarget.3046
中图分类号
R73 [肿瘤学];
学科分类号
100214 [肿瘤学];
摘要
Epstein-Barr virus (EBV) infection is a major etiological factor for nasopharyngeal carcinoma (NPC). Several EBV-encoded BART miRNAs have been associated with viral latency, immune escape, cell survival, cell proliferation and apoptosis. Here, we report that EBV-miR-BART7-3p, an EBV-encoded BART miRNA highly expressed in NPC, was correlated with cell-cycle progression in vitro and increased tumor formation in vivo. This viral miRNA stimulated the PTEN/PI3K/Akt pathway and induced c-Myc and c-Jun. Knockdown of PTEN mimicked EBV-miR-BART7-3p-induced tumorigenic phenotype. Based on these results, we conducted a therapeutic experiment by using gold nano-particles (AuNPs) carrying anti-EBV-miR-BART7-3p. Silencing of EBV-miRBART7-3p reduced tumor growth in animal model. We conclude that EBV-miR-BART7-3p favors carcinogenesis, representing a potential target for miRNA-based therapy.
引用
收藏
页码:7838 / 7850
页数:13
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