Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism

被引:674
作者
Velankar, SS [1 ]
Soultanas, P [1 ]
Dillingham, MS [1 ]
Subramanya, HS [1 ]
Wigley, DB [1 ]
机构
[1] Univ Oxford, Sir William Dunn Sch Pathol, Oxford OX1 3RE, England
基金
英国惠康基金;
关键词
D O I
10.1016/S0092-8674(00)80716-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have determined two different structures of PcrA DNA helicase complexed with the same single strand tailed DNA duplex, providing snapshots of different steps on the catalytic pathway. One of the structures is of a complex with a nonhydrolyzable analog of ATP and is thus a "substrate" complex. The other structure contains a bound sulphate ion that sits in a position equivalent to that occupied by the phosphate ion produced after ATP hydrolysis, thereby mimicking a "product" complex. In both complexes, the protein is monomeric. Large and distinct conformational changes occur on binding DNA and the nucleotide cofactor. Taken together, these structures provide evidence against an "active rolling" model for helicase action but are instead consistent with an "inchworm" mechanism.
引用
收藏
页码:75 / 84
页数:10
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