Antibiotic susceptibility patterns and clones of Pseudomonas aeruginosa in Swedish ICUs

被引:5
作者
Erlandsson, Marcus [1 ]
Gill, Hans [2 ]
Nordlinder, David [1 ]
Giske, Christian G. [3 ]
Jonas, Daniel [4 ,5 ]
Nilsson, Lennart E. [6 ]
Walther, Sten [7 ]
Hanberger, Hakan [1 ]
机构
[1] Linkoping Univ, Fac Hlth Sci, Dept Clin & Expt Med, Div Infect Dis, Linkoping, Sweden
[2] Linkoping Univ, Dept Biomed Engn, Linkoping, Sweden
[3] Karolinska Univ Hosp, Div Clin Microbiol, Stockholm, Sweden
[4] Univ Med Ctr, Inst Environm Med, Freiburg, Germany
[5] Univ Med Ctr, Hosp Epidemiol, Freiburg, Germany
[6] Linkoping Univ, Fac Hlth Sci, Dept Clin & Expt Med, Div Clin Microbiol, Linkoping, Sweden
[7] Univ Oslo, Ulleval Univ Hosp, Dept Anaesthesiol, Oslo, Norway
关键词
D O I
10.1080/00365540701864641
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
Pseudomonas aeruginosa is 1 of the bacteria most adaptive to anti-bacterial treatment. Previous studies have shown nosocomial spread and transmission of clonal strains of P. aeruginosa in European hospitals. In this study we investigated antibiotic susceptibility and clonality in 101 P. aeruginosa isolates from 88 patients admitted to 8 Swedish ICUs during 2002. We also compared phenotypes and genotypes of P. aeruginosa and carried out cluster analysis to determine if phenotypic data can be used for surveillance of clonal spread. All isolates were collected on clinical indication as part of the NPRS II study in Sweden and were subjected to AFLP analysis for genotyping. 68 isolates with unique genotypes were found. Phenotyping was performed using MIC values for 5 anti-pseudomonal agents. Almost 6% of the isolates were multi-drug resistant (MDR), and this figure rose to almost 8% when intermediate isolates were also included. We found probable clonal spread in 9 cases, but none of them was found to be an MDR strain. Phenotypical cluster analysis produced 40 clusters. Comparing partitions did not demonstrate any significant concordance between the typing methods. The conclusion of our study is that cross-transmission and clonal spread of MDR P. aeruginosa does not present a clinical problem in Swedish ICUs, but probable cross-transmission of non-MDR clones indicate a need for improved hygiene routines bedside. The phenotype clusters were not concordant with genotype clusters, and genotyping is still recommended for epidemiological tracking.
引用
收藏
页码:487 / 494
页数:8
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