Recent advances using green and red fluorescent protein variants

被引：83

作者：

Mueller-Taubenberger, Annette

Anderson, Kurt I.

机构：

[1] Univ Munich, Inst Zellbiol, D-80336 Munich, Germany

[2] Beatson Inst Canc Res, Glasgow G61 1BD, Lanark, Scotland

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2007年 / 77卷 / 01期

关键词：

BiFC; fluorescent protein; FRET; GFP; microscopy; RFP; photoactivation; two-photon;

D O I：

10.1007/s00253-007-1131-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Fluorescent proteins have proven to be excellent tools for live-cell imaging. In addition to green fluorescent protein (GFP) and its variants, recent progress has led to the development of monomeric red fluorescent proteins (mRFPs) that show improved properties with respect to maturation, brightness, and the monomeric state. This review considers green and red spectral variants, their paired use for live-cell imaging in vivo, in vitro, and in fluorescence resonance energy transfer (FRET) studies, in addition to other recent "two-color" advances including photoswitching and bimolecular fluorescence complementation (BiFC). It will be seen that green and red fluorescent proteins now exist with nearly ideal properties for dual-color microscopy and FRET.

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页数：12

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