In vitro cortical neuronal networks as a new high-sensitive system for biosensing applications

被引:60
作者
Martinoia, S
Bonzano, L
Chiappalone, A
Tedesco, A
Marcoli, A
Maura, G
机构
[1] Univ Genoa, Dept Biophys & Elect Engn, Neuroengn & Bionanotechnol Grp, I-16145 Genoa, Italy
[2] Univ Genoa, Dept Expt Med, Pharmacol & Toxicol Sect, Genoa, Italy
关键词
in vitro cortical neurons; microelectrode arrays; electrophysiological monitoring; cell-based biosensor; neuropharmacological investigations;
D O I
10.1016/j.bios.2004.09.012
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
By taking advantages of the main features of the microelectrode array (MEA) technology (i.e. multisite recordings, stable and long-term coupling with the biological preparation), we analyzed the changes in activity patterns induced by applying specific substances to dissociated cortical neurons from rat-embryos (E18). Data were recorded simultaneously from 60 electrodes, and the electrophysiological behavior was investigated during the third week in vitro, both at the spike and burst level. The analysis of the electrophysiological activity modulation, by applying agonists of the ionotropic glutamate receptors at low (i.e. 0.2-1-5 mu M) and high (i.e. 50-100 mu M) concentrations, is presented. Preliminary results show that the dynamics of the in vitro cortical neurons is very sensitive to pharmacological manipulation of the glutamatergic transmission and the effects on the network behavior are strictly dependent from the drug concentration. In particular, the addition of a high-dose of agonist determined a global and irreversible depression of the network activity, while, in the low-concentration case, the electrophysiological behavior showed different results, depending on the type of receptor involved. From these observations, we are encouraged to think of a more engineered system, based on in vitro cortical neurons, as a novel sensitive system for drug (pre)-screening and neuropharmacological evaluations. (c) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码:2071 / 2078
页数:8
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