Investigation of the cell surface structures of agrobacteria involved in bacterial and plant interactions

被引:3
作者
Chumakov, MI [1 ]
Dykman, LA [1 ]
Bogatyrev, VA [1 ]
Kurbanova, IV [1 ]
机构
[1] Russian Acad Sci, Inst Biochem & Physiol Plants & Microorganisms, Saratov 410015, Russia
基金
俄罗斯基础研究基金会;
关键词
agrobacteria; VirB2; protein; microfibrils;
D O I
10.1023/A:1010446000969
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Agrobacterial cells produced straight microfibrils not only when in contact with wheat seedling roots, but also when in contact with each other. After 2 h of incubation, agrobacterial cells were found to form aggregates, in which the cells were in contact either directly or through thick straight microfibrils (bridges) of an unknown composition. The majority of the microfibrils were susceptible to attack by cellulase, although some of them showed resistance to this enzyme. Like the wild-type flagellated agrobacteria, their bald mutants produced long straight microfibrils. The cell surface structures of agrobacteria were examined by labeling them immunocytochemically with colloidal gold-conjugated antibodies against O-specific lipopolysaccharides, Vir proteins, and cellulase. Agrobacterial cells treated with acetosyringone and brought into contact were found to contain subpolar and polar cell surface structures. Antibodies against the VirB2 protein were able to interact with a tuft of thin microfibrils located on one pole of the agrobacterial cell whose vir genes were induced by acetosyringone but were unable to interact with the surface structures of the agrobacterial cells aggregated in liquid medium in the absence of wheat seedlings.
引用
收藏
页码:232 / 238
页数:7
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