LC-NMR and LC-MS analysis of 2,3,10,11-oxygenated protoberberine metabolites in Corydalis cell cultures

被引：17

作者：

Iwasa, K

Kuribayashi, A

Sugiura, M

Moriyasu, M

Lee, DU

Wiegrebe, W

机构：

[1] Kobe Pharmaceut Univ, Higashinada Ku, Kobe, Hyogo 6588558, Japan

[2] Dongguk Univ, Coll Nat Sci, Dept Biochem, Kyongju 780714, South Korea

[3] Univ Regensburg, Inst Pharm, D-93040 Regensburg, Germany

来源：

PHYTOCHEMISTRY | 2003年 / 64卷 / 07期

关键词：

Corydalis platycarpa; Corydalis ochotensis var. raddeana; Fumariaceae; cell cultures; biotransformation; LC-MS; LC-NMR; secondary metabolism; protoberberine alkaloids;

D O I：

10.1016/S0031-9422(03)00497-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The metabolism of 2,3, 10,1 I-oxygenated protoberberine alkaloids was studied in cell cultures of Corydalis species. Without prior isolation, the structures of the metabolites were determined by LC-MS and LC-NMR analyses. Tetrahydropseudocoptisine alpha-N-metho salt, pseudoprotopine, and pseudomuramine were identified for the first time, and preliminary evidence for metabolic pathways to the formation of these alkaloids were obtained. (C) 2003 Elsevier Ltd. All rights reserved.

引用

页码：1229 / 1238

页数：10

共 12 条

[1]

Hostettmann K, 1997, PHARM IND, V59, P339

[2] Structure-activity relationships of quaternary protoberberine alkaloids having an antimalarial activity [J].