Influenza virus (A/HK/156/97) hemagglutinin expressed by an alphavirus replicon system protects chickens against lethal infection with Hong Kong-origin H5N1 viruses

被引：81

作者：

Schultz-Cherry, S

Dybing, JK

Davis, NL

Williamson, C

Suarez, DL

Johnston, R

Perdue, ML

机构：

[1] ARS, SE Poultry REs Lab, USDA, Athens, GA 30605 USA

[2] Univ N Carolina, Dept Microbiol & Immunol, Chapel Hill, NC USA

来源：

VIROLOGY | 2000年 / 278卷 / 01期

关键词：

D O I：

10.1006/viro.2000.0635

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Venezuelan equine encephalitis virus replicon particles (VRP) containing the gene expressing hemagglutinin (HA) from the human Hong Kong Influenza A isolate (A/HK/156/97) were evaluated as vaccines in chicken embryos and young chicks. Expressed HA was readily detected in bird-tissue staining with anti-H5 HA antibody and in chicken cells infected with the replicon preparations following immunoprecipitation with monoclonal antibody. Birds challenged with a dose of the lethal parent virus were protected to different extents depending on the age of the bird. in ovo and 1-day-old inoculated animals that received no boost with the VRP were partially protected; birds 2 weeks of age were completely protected with a single dose of VRP. (C) 2000 Academic Press.

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页码：55 / 59

页数：5

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