Microplate-array diagonal-gel electrophoresis (MADGE) and melt-MADGE: tools for molecular-genetic epidemiology

被引：11

作者：

Day, INM ^{[1
]}

Spanakis, E ^{[1
]}

Palamand, D ^{[1
]}

Weavind, GP ^{[1
]}

O'Dell, SD ^{[1
]}

机构：

[1] Southampton Univ Hosp NHS Trust, Wessex Human Genet Inst, Southampton SO16 6YD, Hants, England

来源：

TRENDS IN BIOTECHNOLOGY | 1998年 / 16卷 / 07期

关键词：

D O I：

10.1016/S0167-7799(98)01217-7

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Microplate-array diagonal-gel electrophoresis (MADGE) was invented for molecular-genetic epidemiological studies. It combines direct compatibility with microplates, convenient polyacrylamide-gel electrophoresis and economy of time and reagents at minimal capital cost, and enables one user to run up to several-thousand gel lanes per day for the direct assay of single-base variations. Melt-MADGE adds temporal thermal-ramp apparatus to achieve similar throughput for de novo mutation scanning.

引用

页码：287 / 290

页数：4

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