Kupffer cell heterogeneity: functional properties of bone marrow-derived and sessile hepatic macrophages

被引:258
作者
Klein, Ingo
Cornejo, Judith C.
Polakos, Noelle K.
John, Beena
Wuensch, Sherry A.
Topham, David J.
Pierce, Robert H.
Crispe, Ian Nicholas
机构
[1] Univ Rochester, Sch Med & Dent, David Smith Ctr Vaccine Biol Immunol, Aab Inst Biomed Res, Rochester, NY USA
[2] Univ Wurzburg, Dept Gen Surg, Wurzburg, Germany
[3] Univ Rochester, Sch Med & Dent, Dept Pathol, Rochester, NY 14627 USA
关键词
D O I
10.1182/blood-2007-02-073841
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Kupffer cells form a large intravascular macrophage bed in the liver sinusoids. The differentiation history and diversity of Kupffer cells is disputed; some studies argue that they are derived from blood monocytes, whereas others support a local origin from intrahepatic precursor cells. In the present study, we used both flow cytometry and immunohistochemistry to distinguish 2 subsets of Kupffer cells that were revealed in the context both of bone marrow transplantation and of orthotopic liver transplantation. One subset was radiosensitive and rapidly replaced from hematogenous precursors, whereas the other was relatively radioresistant and long-lived. Both were phagocytic but only the former population was recruited into inflammatory foci in response to CD8+ T-cell activation. We propose the name "sessile" for the radioresistant Kupffer cells that do not partici-pate in immunoinflammatory reactions. However, we found no evidence that these sessile Kupffer cells arise from immature intrahepatic precursors. Our conclusions resolve a long-standing controversy and explain how different experimental approaches may reveal one or both of these subsets.
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页码:4077 / 4085
页数:9
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