Chikusetsu saponin V attenuates H2O2-induced oxidative stress in human neuroblastoma SH-SY5Y cells through Sirt1/PGC-1α/Mn-SOD signaling pathways

被引：44

作者：

Wan, Jingzhi ^{[1
]}

Deng, Lili ^{[1
]}

Zhang, Changcheng ^{[1
]}

Yuan, Qin ^{[1
]}

Liu, Jing ^{[1
]}

Dun, Yaoyan ^{[1
]}

Zhou, Zhiyong ^{[1
]}

Zhao, Haixia ^{[1
]}

Liu, Chaoqi ^{[2
]}

Yuan, Ding ^{[3
]}

Wang, Ting ^{[1
]}

机构：

[1] Three Gorges Univ, Coll Med Sci, Yichang 443002, Hubei, Peoples R China

[2] Three Gorges Univ, Inst Mol Biol, Yichang 443002, Hubei, Peoples R China

[3] Three Gorges Univ, Renhe Hosp, Coll Clin Med Sci 2, Yichang 443001, Hubei, Peoples R China

来源：

CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY | 2016年 / 94卷 / 09期

关键词：

Chikusetsu saponin V; SH-SY5Y cells; Sirt1; PGC-1; alpha; Mn-SOD; PANAX-JAPONICUS; SIRT1; DAMAGE; APOPTOSIS; DEATH; RESPIRATION; PARKINSONS; METABOLISM; EXTRACT; BRAIN;

D O I：

10.1139/cjpp-2015-0262

中图分类号：

R9 [药学];

学科分类号：

100702 [药剂学];

摘要：

Oxidative stress plays a vital role in the pathogenesis of neurodegenerative diseases. Chikusetsu saponin V (CsV), the most abundant member of saponins from Panax japonicus (SPJ), has attracted increasing attention for its potential to treat neurodegenerative diseases. However, the mechanisms are unclear. Our study intended to investigate the antioxidative effects of CsV in human neuroblastoma SH-SY5Y cells. Our data showed that CsV attenuated H2O2-induced cytotoxicity, inhibited ROS accumulation, increased the activities of superoxide dismutase (SOD) and GSH, and increased mitochondrial membrane potential dose-dependently. Further exploration of the mechanisms showed that CsV exhibited these effects through increasing the activation of oxidative-stress-associated factors including Sirt1, PGC-1 alpha, and Mn-SOD. Moreover, CsV inhibited H2O2-induced down-regulation of Bcl-2 and up-regulation of Bax in a dose-dependent manner and, thus, increased the ratio of Bcl-2/Bax. In conclusion, our study demonstrated that CsV exhibited neuroprotective effects possibly through Sirt1/PGC-1 alpha/Mn-SOD signaling pathways.

引用

页码：919 / 928

页数：10

共 34 条

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