Decreased contents of surfactant proteins A and D in BAL fluids of healthy smokers

被引:166
作者
Honda, Y
Takahashi, H
Kuroki, Y
Akino, T
Abe, S
机构
[1] SAPPORO MED UNIV,SCH MED,DEPT INTERNAL MED 3,SAPPORO,HOKKAIDO,JAPAN
[2] SAPPORO MED UNIV,SCH MED,DEPT BIOCHEM,SAPPORO,HOKKAIDO,JAPAN
关键词
bronchoalveolar lavage fluid; pulmonary surfactant; smoking; surfactant protein A; surfactant protein D;
D O I
10.1378/chest.109.4.1006
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
Hydrophilic surfactant proteins, surfactant protein A (SP-A) and surfactant protein D (SP-D), have important roles in modulating the host defense functions in the peripheral airways. It has been reported that cigarette smoke may alter the component and function of pulmonary surfactant, In this study, we determined the contents of SP-A and SP-D in BAL fluids of healthy smokers and nonsmokers by enzyme-linked immunosorbent assay using monoclonal antibodies against each protein. The contents of SP-A and SP-D in BAL fluids were significantly (p<0.05) decreased in smokers compared to those in nonsmokers, although there was no significant difference of total phospholipid content between smokers and nonsmokers. These results suggest that the decreased levels of SP-A and SP-D in smokers may impair the host defense functions of surfactant in the peripheral airways and might have a crucial role in the development of chronic obstructive lung disease.
引用
收藏
页码:1006 / 1009
页数:4
相关论文
共 31 条
[1]  
BARTLETT GR, 1959, J BIOL CHEM, V234, P466
[2]  
BLIGH EG, 1959, CAN J BIOCHEM PHYS, V37, P911
[3]   EFFECT OF SURFACTANT-ASSOCIATED PROTEIN-A (SP-A) ON THE ACTIVITY OF LIPID EXTRACT SURFACTANT [J].
CHUNG, J ;
YU, SH ;
WHITSETT, JA ;
HARDING, PGR ;
POSSMAYER, F .
BIOCHIMICA ET BIOPHYSICA ACTA, 1989, 1002 (03) :348-358
[4]  
Cook W A, 1966, Ann Thorac Surg, V2, P327
[5]   LOW YIELD OF PULMONARY SURFACTANT IN CIGARETTE SMOKERS [J].
FINLEY, TN ;
LADMAN, AJ .
NEW ENGLAND JOURNAL OF MEDICINE, 1972, 286 (05) :223-&
[6]  
FINLEY TN, 1983, PULMONARY SURFACTANT, P297
[7]  
GIAMMONA ST, 1967, AM REV RESPIR DIS, V96, P539
[8]  
HAAGSMAN HP, 1991, ANNU REV PHYSIOL, V53, P441, DOI 10.1146/annurev.ph.53.030191.002301
[9]   ELEVATED SURFACTANT PROTEIN-A IN BRONCHOALVEOLAR LAVAGE FLUIDS FROM SARCOIDOSIS AND HYPERSENSITIVITY PNEUMONITIS PATIENTS [J].
HAMM, H ;
LUHRS, J ;
ROTAECHE, JGY ;
COSTABEL, U ;
FABEL, H ;
BARTSCH, W .
CHEST, 1994, 106 (06) :1766-1770
[10]   PULMONARY SURFACTANT PROTEIN-D IN SERA AND BRONCHOALVEOLAR LAVAGE FLUIDS [J].
HONDA, Y ;
KUROKI, Y ;
MATSUURA, E ;
NAGAE, H ;
TAKAHASHI, H ;
AKINO, T ;
ABE, S .
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 1995, 152 (06) :1860-1866