Coupling between B cell receptor and phospholipase C-γ2 is essential for mature B cell development

被引：47

作者：

Hikida, M

Johmura, S

Hashimoto, A

Takezaki, M

Kurosaki, T ^{[1
]}

机构：

[1] Kansai Med Univ, Dept Mol Genet, Inst Liver Res, Moriguchi, Osaka 5708506, Japan

[2] RIKEN, Res Ctr Allergy & Immunol, Lab Lymphocyte Differentiat, Moriguchi, Osaka 5708506, Japan

来源：

JOURNAL OF EXPERIMENTAL MEDICINE | 2003年 / 198卷 / 04期

关键词：

cell differentiation; cell survival; B lymphocyte subsets; spleen; tumor necrosis factor;

D O I：

10.1084/jem.20030280

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Two signaling pathways known to be essential for progression from miniature to mature B cells are BAFF receptor (BAFF-R) and the B cell receptor (BCR). Here, we first show that phospholipase C (PLC)-gamma2 is required for a BAFF-R-mediated survival signal. Then, we have examined the question of whether the reduced number of mature B cells in PLC-gamma2(-/-) mice is caused by a defect in either BCP, or BAFF-R signaling. We find that a PLC-gamma2 SH2 mutant, which inhibits coupling between BCR and PLC-gamma2, fails to restore B cell maturation, despite supporting BAFF-dependent survival. Therefore, our data suggest that the BAFF-R-mediated survival signal, provided by PLC-gamma2, is not sufficient to promote B cell maturation, and that, in addition, activation of PLC-gamma2 by BCR is required for B cell development.

引用

收藏

页码：581 / 589

页数：9

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