Acting on actin:: the electric motility assay

被引：118

作者：

Riveline, D

Ott, A

Jülicher, F

Winkelmann, DA

Cardoso, O

Lacapère, JJ

Magnúsdóttir, S

Viovy, JL

Gorre-Talini, L

Prost, J

机构：

[1] CNRS, Lab Phys Chim Curie, Inst Curie, Sect Rech, F-75231 Paris 05, France

[2] Univ Paris 07, Lab Phys Chim Curie, Inst Curie, Sect Rech, F-75231 Paris, France

[3] Univ Med & Dent New Jersey, Robert Wood Johnson Med Sch, Dept Pathol, Piscataway, NJ 08854 USA

[4] Ecole Normale Super, Phys Stat Lab, CNRS, F-75231 Paris 05, France

[5] Univ Paris 06, Phys Stat Lab, CNRS, Ecole Normale Super, F-75231 Paris 05, France

[6] Univ Paris 07, Phys Stat Lab, CNRS, Ecole Normale Super, F-75231 Paris 05, France

来源：

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS | 1998年 / 27卷 / 04期

关键词：

actin; myosin; electric fields PMMA; instability;

D O I：

10.1007/s002490050147

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

We have developed a novel technique which allows one to direct the two dimensional motion of actin filaments on a myosin coated sheet using a weak electric field parallel to the plane of motion. The filament velocity can be increased or decreased, and even reversed, as a function of orientation and strength of the field. PMMA (poly(methylmethacrylate)) gratings, which act as rails for actin, allow one for the first time to explore three quadrants of the force velocity diagram. We discuss effective friction, duty ratio and stall force at different myosin densities. A discontinuity in the velocity force relationship suggests the existence of dynamical phase transition.

引用

页码：403 / 408

页数：6

共 25 条

[1] SPIRAL DEFECTS IN MOTILITY ASSAYS - A MEASURE OF MOTOR PROTEIN FORCE [J].