Decreased meiotic reciprocal recombination in subtelomeric regions in Saccharomyces cerevisiae

被引:18
作者
Su, YP [1 ]
Barton, AB [1 ]
Kaback, DB [1 ]
机构
[1] Univ Med & Dent New Jersey, New Jersey Med Sch, Dept Microbiol & Mol Genet, Newark, NJ 07103 USA
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
D O I
10.1007/s004120000098
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Reciprocal recombination (chiasma formation) between homologs appears to be essential for promoting chromosome segregation at the first meiotic division. However, chiasmata that form near the ends of chromosomes are much less efficient at promoting segregation. To determine the frequency of reciprocal recombination near the end of a chromosome, genetic markers were inserted at similar to7 kb intervals within the leftmost 30 kb of chromosome I from Saccharomyces cerevisiae. Analysis of recombination between the markers indicated that meiotic reciprocal recombination rates were much lower than on the rest of the chromosome and that rates increased with distance from the telomere. Thus, S. cerevisiae has evolved a mechanism that minimizes the occurrence of chiasmata that cannot promote meiotic segregation. Low rates of recombination were independent of the SIR2 and SIR3 gene products, suggesting that any mechanism for suppressing recombination was different from transcriptional repression due to a telomere position effect.
引用
收藏
页码:467 / 475
页数:9
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