Effect of methylprednisolone and mechanical loading on canine articular cartilage in explant culture

被引:24
作者
Farquhar, T
Todhunter, RJ
Fubini, SL
BurtonWurster, N
Lust, G
机构
[1] Department Mechanical Engineering, University Maryland Baltimore County, Baltimore
关键词
cartilage; corticosteroids; methylprednisolone; articular load;
D O I
10.1016/S1063-4584(96)80007-0
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
The objective of this study was to assess the effect of mechanical load on articular cartilage after in vitro corticosteroid exposure. Canine humeral cartilage was equilibrated for 4 days in defined medium with a serum substitute, then exposed to methylprednisolone sodium succinate for 20 h at 0, 0.01 or 1.0 mg/ml. After a drug-free recovery period, the explants were subjected to 0, 1 or 10 mega pascals (MPa) for 1 out every 5 s for 20 min, then incubated with [S-35]-sulfate and [H-3]-leucine for 4 h to measure proteoglycan and protein synthesis, respectively. When the loading occurred 22 h after drug exposure, proteoglycan synthesis was inhibited and protein synthesis was unaffected by the drug. Both were stimulated by load, relative to controls. When the loading was delayed until 142 h after drug exposure, there was no biosynthetic response to load whether or not the explant had been exposed to the drug. Proteoglycan and protein synthesis 142 h after 0 or 0.01 mg/ml were unchanged or slightly higher than at 22 h, in explants which did not receive load. In contrast, biosynthesis were strongly inhibited 142 h after 1.0 mg/ml, and there was a 40% loss of proteoglycan content, relative to 22 h controls. If explants receiving 1.0 mg/ml also received heavy (10 MPa) loads 142 h later, there was a 17% reduction in total dry content suggesting severe matrix damage. These in vitro results suggest that articular load can help maintain normal cartilage metabolism after corticosteroid exposure, but also suggest that heavy loading after a sub-clinical dose can cause a marked loss of matrix solids.
引用
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页码:55 / 62
页数:8
相关论文
共 33 条
[1]   SYNOVIAL-FLUID AND PLASMA KINETICS OF METHYLPREDNISOLONE AND METHYLPREDNISOLONE ACETATE IN HORSES FOLLOWING INTRAARTICULAR ADMINISTRATION OF METHYLPREDNISOLONE ACETATE [J].
AUTEFAGE, A ;
ALVINERIE, M ;
TOUTAIN, PL .
EQUINE VETERINARY JOURNAL, 1986, 18 (03) :193-198
[2]   ALTERATION OF RABBIT ARTICULAR-CARTILAGE BY INTRA-ARTICULAR INJECTIONS OF GLUCOCORTICOIDS [J].
BEHRENS, F ;
SHEPARD, N ;
MITCHELL, N .
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 1975, A 57 (01) :70-76
[3]   FIBRONECTIN AND PROTEOGLYCAN SYNTHESIS IN LONG-TERM CULTURES OF CARTILAGE EXPLANTS IN HAM F-12 SUPPLEMENTED WITH INSULIN AND CALCIUM - EFFECTS OF THE ADDITION OF TGF-BETA [J].
BURTONWURSTER, N ;
LUST, G .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1990, 283 (01) :27-33
[4]   EVIDENCE FOR A GLYCOSAMINOGLYCAN CHAIN ON A PORTION OF ARTICULAR-CARTILAGE FIBRONECTINS [J].
BURTONWURSTER, N ;
LUST, G .
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1993, 306 (02) :309-320
[5]   REDUCED DEPOSITION OF COLLAGEN IN THE DEGENERATED ARTICULAR-CARTILAGE OF DOGS WITH DEGENERATIVE JOINT DISEASE [J].
BURTONWURSTER, N ;
HUICHOU, CS ;
GREISEN, HA ;
LUST, G .
BIOCHIMICA ET BIOPHYSICA ACTA, 1982, 718 (01) :74-84
[6]  
CHUNEKAMRAI S, 1989, AM J VET RES, V50, P1733
[7]   A DIRECT SPECTROPHOTOMETRIC MICRO-ASSAY FOR SULFATED GLYCOSAMINOGLYCANS IN CARTILAGE CULTURES [J].
FARNDALE, RW ;
SAYERS, CA ;
BARRETT, AJ .
CONNECTIVE TISSUE RESEARCH, 1982, 9 (04) :247-248
[8]  
FARQUHAR T, IN PRESS J ORTH RES
[9]  
FOLAND JW, 1991, 26 ANN ACVS SCI M
[10]  
FUBINI SL, 1993, AM J VET RES, V54, P1359