Formulation of vaccines containing CpG oligonucleotides and alum

被引：52

作者：

Aebig, Joan A. ^{[1
]}

Mullen, Gregory E. D. ^{[1
]}

Dobrescu, Gelu ^{[1
]}

Rausch, Kelly ^{[1
]}

Lambert, Lynn ^{[1
]}

Ajose-Popoola, Olubunmi ^{[1
]}

Long, Carole A. ^{[1
]}

Saul, Allan ^{[1
]}

Miles, Aaron P. ^{[1
]}

机构：

[1] NIAID, Malaria Vaccine Dev Branch, NIH, Rockville, MD 20852 USA

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 2007年 / 323卷 / 02期

关键词：

vaccine; formulation; CPG; 7909; IP-10;

D O I：

10.1016/j.jim.2007.04.003

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

CpG oligodeoxynucleotides are potent immunostimulants. For parenterally delivered alum-based vaccines, the immunostimulatory effect of CpG depends on the association of the CpG and antigen to the alum. We describe effects of buffer components on the binding of CPG 7909 to aluminum hydroxide (Alhydrogel), assays for measuring binding of CPG 7909 to alum and CPG 7909 induced dissociation of antigen from the alum. Free CPG 7909 is a potent inducer of IP-10 in mice. However the lack of IP-10 production from formulations containing bound CPG 7909 suggested that CPG 7909 does not rapidly dissociate from the alum after injection. It also suggests that IP-10 assays are not a good basis for potency assays for alum-based vaccines containing CPG 7909. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：139 / 146

页数：8

共 18 条

[1]

CpG-A-induced monocyte IFN-γ-inducible protein-10 production is regulated by plasmacytoid dendritic cell-derived IFN-α [J].

Blackwell, SE ;

Krieg, AM .

JOURNAL OF IMMUNOLOGY, 2003, 170 (08) :4061-4068

[2]

Immediate-type hypersensitivity and other clinical reactions in volunteers immunized with a synthetic multi-antigen peptide vaccine (PfCS-MAP1NYU) against Plasmodium falciparum sporozoites [J].

Edelman, R ;

Wasserman, SS ;

Kublin, JG ;

Bodison, SA ;

Nardin, EH ;

Oliveira, GA ;

Ansari, S ;

Diggs, CL ;

Kashala, OL ;

Schmeckpeper, BJ ;

Hamilton, RG .

VACCINE, 2002, 21 (3-4) :269-280

[3]

Delineation of a CpG phosphorothioate oligodeoxynucleotide for activating primate immune responses in vitro and in vivo [J].

Hartmann, G ;

Weeratna, RD ;

Ballas, ZK ;

Payette, P ;

Blackwell, S ;

Suparto, I ;

Rasmussen, WL ;

Waldschmidt, M ;

Sajuthi, D ;

Purcell, RH ;

Davis, HL ;

Krieg, AM .

JOURNAL OF IMMUNOLOGY, 2000, 164 (03) :1617-1624

[4]

Effect of the degree of phosphate substitution in aluminum hydroxide adjuvant on the adsorption of phosphorylated proteins [J].

Iyer, S ;

HogenEsch, H ;

Hem, SL .

PHARMACEUTICAL DEVELOPMENT AND TECHNOLOGY, 2003, 8 (01) :81-86

[5]

Kaslow DC, 2002, CHEM IMMUNOL, V80, P287

[6]

In vitro studies with recombinant Plasmodium falciparum apical membrane antigen 1 (AMA1):: Production and activity of an AMA1 vaccine and generation of a multiallelic response [J].

Kennedy, MC ;

Wang, J ;

Zhang, YL ;

Miles, AP ;

Chitsaz, F ;

Saul, A ;

Long, CA ;

Miller, LH ;

Stowers, AW .

INFECTION AND IMMUNITY, 2002, 70 (12) :6948-6960

[7]

Use of CpG oligodeoxynucleotides as immune adjuvants [J].

Klinman, DM ;

Currie, D ;

Gursel, I ;

Verthelyi, D .

IMMUNOLOGICAL REVIEWS, 2004, 199 (01) :201-216

[8]

Induction of systemic TH1-like innate immunity in normal volunteers following subcutaneous but not intravenous administration of CPG 7909, a synthetic B-Class CpG oligodeoxynucleotide TLR9 agonist [J].

Krieg, AM ;

Efler, SM ;

Wittpoth, M ;

Al Adhami, MJ ;

Davis, HL .

JOURNAL OF IMMUNOTHERAPY, 2004, 27 (06) :460-471

[9]

Phase 1 vaccine trial of Pvs25H:: a transmission blocking vaccine for Plasmodium vivax malaria [J].

Malkin, EM ;

Durbin, AP ;

Diemert, DJ ;

Sattabongkot, J ;

Wu, YM ;

Miura, K ;

Long, CA ;

Lambert, L ;

Miles, AP ;

Wang, J ;

Stowers, A ;

Miller, LH ;

Saul, A .

VACCINE, 2005, 23 (24) :3131-3138

[10]

Phase 1 clinical trial of apical membrane antigen 1:: an asexual blood-stage vaccine for Plasmodium falciparum malaria [J].

Malkin, EM ;

Diemert, DJ ;

McArthur, JH ;

Perreault, JR ;

Miles, AP ;

Giersing, BK ;

Mullen, GE ;

Orcutt, A ;

Muratova, O ;

Awkal, M ;

Zhou, H ;

Wang, J ;

Stowers, A ;

Long, CA ;

Mahanty, S ;

Miller, LH ;

Saul, A ;

Durbin, AP .

INFECTION AND IMMUNITY, 2005, 73 (06) :3677-3685

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