Oligonucleotide probes that detect quantitatively significant groups of butyrate-producing bacteria in human feces

被引：269

作者：

Hold, GL

Schwiertz, A

Aminov, RI

Blaut, M

Flint, HJ

机构：

[1] Rowett Res Inst, Aberdeen AB21 9SB, Scotland

[2] Deutsch Inst Ernaehrungsforsch, Abt Gastrointestinale Mikrobiol, D-14558 Bergholz Rehbrucke, Germany

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2003年 / 69卷 / 07期

关键词：

D O I：

10.1128/AEM.69.7.4320-4324.2003

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

16S rRNA-targeted oligonucleotide probes were designed for butyrate-producing bacteria from human feces. Three new cluster-specific probes detected bacteria related to Roseburia intestinalis, Faecalibacterium prausnitzii, and Eubacterium hallii at mean populations of 2.3, 3.8, and 0.6%, respectively, in samples from 10 individuals. Additional species-level probes accounted for no more than 1%, with a mean of 7.7%, of the total human fecal microbiota identified as butyrate producers in this study. Bacteria related to E. hallii and the genera Roseburia and Faecalibacterium are therefore among the most abundant known butyrate-producing bacteria in human feces.

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页码：4320 / 4324

页数：5

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