Simple method for clinical determination of 13 carotenoids in human plasma using an isocratic high-performance liquid chromatographic method

被引：107

作者：

Lyan, B ^{[1
]}

Azaïs-Braesco, V ^{[1
]}

Cardinault, N ^{[1
]}

Tyssandier, V ^{[1
]}

Borel, P ^{[1
]}

Alexandre-Gouabau, MC ^{[1
]}

Grolier, P ^{[1
]}

机构：

[1] INRA, CRNH Ctr Clermont Ferrand Theix, Unite Malad Metab & Micronutriments, F-63122 St Genes Champanelle, France

来源：

JOURNAL OF CHROMATOGRAPHY B | 2001年 / 751卷 / 02期

关键词：

carotenoids;

D O I：

10.1016/S0378-4347(00)00488-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report a reversed-phase high-performance liquid chromatography method which resolves 13 identified carotenoids and nine unknown carotenoids from human plasma. A Nucleosil C-18 column and a Vydac C-18 column in series are used with an isocratic solvent system of acetonitrile-methanol containing 50 mM acetate ammonium-dichloromethane - water (70:15:10:5, v/v/v/v) as mobile phase at a flow-rate of 2 ml/min. The intra-day (4.5-8.3%) and inter-day (1.3-12.7%) coefficients of variation are suitable for routine clinical determinations. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：297 / 303

页数：7

共 25 条

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[2]

Bauernfeind J.C., 1981, CAROTENOIDS COLORANT

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