Selective control of cytosolic glutathione peroxidase and phospholipid hydroperoxide glutathione peroxidase mRNA stability by selenium supply

被引：66

作者：

Bermano, G ^{[1
]}

Arthur, JR ^{[1
]}

Hesketh, JE ^{[1
]}

机构：

[1] ROWETT RES INST,DIV BIOCHEM SCI,BUCKSBURN AB21 9SB,ABERDEEN,SCOTLAND

来源：

FEBS LETTERS | 1996年 / 387卷 / 2-3期

关键词：

messenger RNA; translation; selenium; glutathione peroxidase (rat hepatoma cell); stability; 3'-untranslated region;

D O I：

10.1016/0014-5793(96)00493-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Selenium depletion of H4 hepatoma cells reduced cytosolic glutathione peroxidase (cGSH-Px) mRNA abundance but had no effect on phospholipid hydroperoxide glutathione peroxidase (PHGSH-Px) mRNA abundance, Actinomycin D chase experiments showed that selenium depletion had no effect on the stability of PHGSH-Px mRNA but decreased the stability of cGSH-Px mRNA. In Se-replete cells puromycin decreased the stability of both cGSH-Px and PHGSH-Px mRNAs. The results suggest that when selenium supply is limiting PHGSH-Px mRNA translation is maintained more than that of cGSH-Px mRNA, and thus more cGSH-Px mRNA is released from polysomes and degraded.

引用

页码：157 / 160

页数：4

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