CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell

被引：4

作者：

Aguiar, D. P. ^{[1
]}

Coelho-Aguiar, J. M. ^{[1
]}

Abreu, J. G. ^{[1
]}

机构：

[1] Univ Fed Rio de Janeiro, Inst Ciencias Biomed, Programa Biol Celular & Desenvolvimento, BR-21949590 Rio de Janeiro, Brazil

来源：

BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH | 2011年 / 44卷 / 03期

关键词：

Teratocarcinoma; Adhesion; Condensation; CCN family; CCN2/CTGF; Extracellular matrix; TISSUE-GROWTH-FACTOR; FACTOR CTGF; TRANSCRIPTION FACTOR; BREAST-CANCER; RETINOIC ACID; UP-REGULATION; IN-VITRO; DIFFERENTIATION; MIGRATION; ANGIOGENESIS;

D O I：

10.1590/S0100-879X2011007500019

中图分类号：

Q [生物科学];

学科分类号：

090105 [作物生产系统与生态工程];

摘要：

Connective tissue growth factor (CCN2/CTGF) is a matricellular-secreted protein involved in extracellular matrix remodeling. The P19 cell line is an embryonic carcinoma line widely used as a cellular model for differentiation and migration studies. In the present study, we employed an exogenous source of CCN2 and small interference RNA to address the role of CCN2 in the P19 cell aggregation phenomenon. Our data showed that increasing CCN2 protein concentrations from 0.1 to 20 nM decreased the number of cell clusters and dramatically increased cluster size without changing proliferation or cell survival, suggesting that CCN2 induced aggregation. In addition, CCN2 specific silencing inhibited typical P19 cell aggregation, which could be partially rescued by 20 nM CCN2. The present study demonstrates that CCN2 is a key molecule for cell aggregation of embryonic P19 cells.

引用

页码：200 / 205

页数：6

共 40 条

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