Quantification of the influenza virus load by real-time polymerase chain reaction in nasopharyngeal swabs of patients treated with oseltamivir

被引：32

作者：

Boivin, G

Coulombe, Z

Wat, C

机构：

[1] CHUQ CHUL, Res Ctr Infect Dis, Quebec City, PQ G1V 4G2, Canada

[2] Univ Laval, Quebec City, PQ, Canada

[3] Roche Global Dev, Welwyn Garden City, Herts, England

来源：

JOURNAL OF INFECTIOUS DISEASES | 2003年 / 188卷 / 04期

关键词：

D O I：

10.1086/377046

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Influenza A virus load was assessed by real-time polymerase chain reaction (PCR) in nasopharyngeal swabs of infected patients treated with oseltamivir. The mean pretreatment virus load was significantly lower in the 24 patients ( group A) who initiated treatment within 24 h of the onset of symptoms than it was in the 26 patients ( group B) who initiated treatment between 24 and 48 h (1.6 x 10(5) vs. 8.4 x 10(5) copies/600 ng of total RNA, P = .04); after 48 h of treatment, twice as many patients in group B still had a positive PCR result, compared with patients in group A (42.3% vs. 20.7%). These virological results support the clinical benefit provided by early therapeutic intervention of influenza illness.

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收藏

页码：578 / 580

页数：3

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