Introducing StuI sites improves vectors for the expression of fusion proteins with factor Xa cleavage sites

被引：11

作者：

Gorlach, J ^{[1
]}

Schmid, J ^{[1
]}

机构：

[1] ETH ZURICH,INST PLANT SCI,CH-8092 ZURICH,SWITZERLAND

来源：

GENE | 1996年 / 170卷 / 01期

关键词：

Escherichia coli; expression vector; factor Xa; recombinant DNA; fusion protein;

D O I：

10.1016/0378-1119(95)00825-X

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The Arg-encoding triplet (AGG) in the recognition sequence Ile-Glu-Gly-Arg for factor Xa can be used to generate a StuI restriction site (AGGCCT) which greatly facilitates the construction of DNA fragments encoding fusion proteins. Following proteolytic cleavage with factor Xa, a protein with the desired N terminus can be obtained.

引用

页码：145 / 146

页数：2

共 4 条

[1] STRUCTURAL AND CATALYTIC PROPERTIES OF THE 4 PHENYLALANINE AMMONIA-LYASE ISOENZYMES FROM PARSLEY (PETROSELINUM-CRISPUM NYM) [J].