Simultaneous Th1-type cytokine expression is a signature of peritoneal CD4+ lymphocytes responding to infection with Listeria monocytogenes

被引:12
作者
Freeman, MM [1 ]
Ziegler, HK [1 ]
机构
[1] Emory Univ, Sch Med, Dept Microbiol & Immunol, Atlanta, GA 30322 USA
关键词
D O I
10.4049/jimmunol.175.1.394
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The robust murine response to infection with Listeria monocytogenes makes an excellent model to study the functional development of immune cells. We investigated the cellular immune response to i.p. infection using intracellular cytokine staining to identify Ag-specific lymphocytes. CD4(+) peritoneal exudate cells obtained 10 days postinfection predominantly coexpressed TNF-alpha, IFN-gamma, and IL-2 after polyclonal or Ag stimulation. A population of cells simultaneously making TNF-alpha and IFN-gamma was also detected but at a lower frequency. By following the kinetics of the response to Listeria, we found that CD4(+) lymphocytes coexpressing TNF-alpha and IFN-gamma dominated on day 6 postinfection and then declined. From days 10-27, TNF-alpha+IFN-gamma+IL-2(+) (triple-positive) was the most prevalent cytokine phenotype, and the frequency steadily declined. These characteristic cytokine expression patterns were observed in both primary and secondary responses to Listeria infection and developed even when infection was terminated with antibiotic treatment. A cytokine-assisted immunization procedure resulted in both double- and triple-positive cells, but the clear predominance of triple-positive cells required Listeria infection. Triple-positive cells were preferentially noted in the peritoneal cavity tissue site; spleen cells displayed a predominant population of double-positive T cells (TNF-alpha+IFN-gamma(+)). We speculate that the appearance of triple-positive cells represents a functionally significant subset important in host defense at nonlymphoid tissue sites.
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收藏
页码:394 / 403
页数:10
相关论文
共 50 条
[1]   REQUIREMENT OF ENDOGENOUS INTERFERON-GAMMA PRODUCTION FOR RESOLUTION OF LISTERIA-MONOCYTOGENES INFECTION [J].
BUCHMEIER, NA ;
SCHREIBER, RD .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (21) :7404-7408
[2]   T lymphocyte dynamics during Listeria monocytogenes infection [J].
Busch, DH ;
Pamer, EG .
IMMUNOLOGY LETTERS, 1999, 65 (1-2) :93-98
[3]   The source of early IFN-γ that plays a role in Th1 priming [J].
Das, G ;
Sheridan, S ;
Janeway, CA .
JOURNAL OF IMMUNOLOGY, 2001, 167 (04) :2004-2010
[4]   IL-2 induces a competitive survival advantage in T lymphocytes [J].
Dooms, H ;
Kahn, E ;
Knoechel, B ;
Abbas, AK .
JOURNAL OF IMMUNOLOGY, 2004, 172 (10) :5973-5979
[5]   A novel approach of direct ex vivo epitope mapping identifies dominant and subdominant CD4 and CD8 T cell epitopes from Listeria monocytogenes [J].
Geginat, G ;
Schenk, S ;
Skoberne, M ;
Goebel, W ;
Hof, H .
JOURNAL OF IMMUNOLOGY, 2001, 166 (03) :1877-1884
[6]   CD8 T-CELLS CAN PROTECT AGAINST AN INTRACELLULAR BACTERIUM IN AN INTERFERON-GAMMA-INDEPENDENT FASHION [J].
HARTY, JT ;
SCHREIBER, RD ;
BEVAN, MJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (23) :11612-11616
[7]   SPECIFIC IMMUNITY TO LISTERIA-MONOCYTOGENES IN THE ABSENCE OF IFN-GAMMA [J].
HARTY, JT ;
BEVAN, MJ .
IMMUNITY, 1995, 3 (01) :109-117
[8]  
HENDRIKS W, 1993, SCIENCE, V259, P1742
[9]   Inhibition of interferon γ induced interleukin 12 production:: A potential mechanism for the anti-inflammatory activities of tumor necrosis factor [J].
Hodge-Dufour, J ;
Marino, MW ;
Horton, MR ;
Jungbluth, A ;
Burdick, RD ;
Strieter, RM ;
Noble, PW ;
Hunter, CA ;
Puré, E .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (23) :13806-13811
[10]   Intestinal and splenic T cell responses to enteric Listeria monocytogenes infection:: Distinct repertoires of responding CD8 T lymphocytes [J].
Huleatt, JW ;
Pilip, I ;
Kerksiek, K ;
Pamer, EG .
JOURNAL OF IMMUNOLOGY, 2001, 166 (06) :4065-4073