Hypoxia increases the association of 4E-binding protein 1 with the initiation factor 4E in isolated rat hepatocytes

被引：53

作者：

Tinton, SA ^{[1
]}

Buc-Calderon, PM ^{[1
]}

机构：

[1] Univ Catholique Louvain, Unite Biochim Toxicol & Cancerol, B-1200 Brussels, Belgium

来源：

FEBS LETTERS | 1999年 / 446卷 / 01期

关键词：

hypoxia; protein synthesis; phosphoprotein heat- and acid-stable induced by insulin; eukaryotic initiation factor;

D O I：

10.1016/S0014-5793(99)00185-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Incubation of hepatocytes under hypoxia increases binding of translation initiation factor eIF-4E to its inhibitory regulator 4E-BP1, and this correlates with dephosphorylation of 4E-BP1. Rapamycin induced the same effect in aerobic cells but no additive effect was observed when hypoxic cells were treated with rapamycin. This enhanced association of 4E-BP1 with eIF-4E might be mediated by mTOR. Nevertheless, only hypoxia produces a rapid inhibition of protein synthesis. Although hypoxia might be signalling via the rapamycin-sensitive pathway by changing eIF-4E availability, such a pathway is unlikely to be responsible for the depression in overall protein synthesis under hypoxia. (C) 1999 Federation of European Biochemical Societies.

引用

页码：55 / 59

页数：5

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