RepA negatively autoregulates the transcription of the repABC operon of the Rhizobium etli symbiotic plasmid basic replicon

被引:34
作者
Ramírez-Romero, MA
Téllez-Sosa, J
Barrios, H
Pérez-Oseguera, A
Rosas, V
Cevallos, MA
机构
[1] Univ Nacl Autonoma Mexico, Ctr Invest Fijac Nitrogeno, Programa Evoluc Mol, Cuernavaca 62191, Morelos, Mexico
[2] Univ Nacl Autonoma Mexico, Inst Biotecnol, Dept Biol Estructural, Cuernavaca 62191, Morelos, Mexico
关键词
D O I
10.1046/j.1365-2958.2001.02621.x
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The basic replicon of Rhizobium etli CE3, like other members of the repABC plasmid family, is constituted by the repABC operon. RepC is essential for replication, and RepA and RepB play a role in plasmid segregation. It has been shown that deletion derivatives lacking the repAB genes have an increased copy number, indicating that these genes participate in the control of plasmid copy number. RepA is also a trans-incompatibility factor. To understand the regulation of the repABC operon, in this paper: (i) the transcription start site of the repABC operon was determined; (I!) the promoter region was identified by site-directed mutagenesis of the putative -35 and -10 hexameric elements; and (iii) RepA was recognized as a negative regulator of the transcription of the repABC operon.
引用
收藏
页码:195 / 204
页数:10
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