Purification of primordial germ cells from TNAP(beta-geo) mouse embryos using FACS-gal

被引：30

作者：

Abe, K

Hashiyama, M

MacGregor, G

Yamamura, K

Abe, K

机构：

[1] KUMAMOTO UNIV,GRAD SCH SCI & TECHNOL,KUMAMOTO 860,JAPAN

[2] EMORY UNIV,SCH MED,DEPT MOL & MED GENET,ATLANTA,GA 30322

来源：

DEVELOPMENTAL BIOLOGY | 1996年 / 180卷 / 02期

基金：

日本科学技术振兴机构;

关键词：

D O I：

10.1006/dbio.1996.0320

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Tissue nonspecific alkaline phosphatase (TNAP), the product of the Akp2 locus, is expressed in mouse primordial germ cells (PGC) for an extensive period during embryogenesis. Mice with the Akp2(tm/Sor) mutant allele of TNAP express lacZ (beta-galactosidase; beta-gal) under control of the Akp2 locus. PGCs were purified from Akp2(tm/Sor) embryos using fluorescence activated cell sorting of beta-gal expressing cells (FACS-gal), Analysis of the purified cells by alkaline phosphatase staining and immunocytochemistry with anti-c-kit antibody demonstrated that highly (98%) purified PGCs can be isolated using this method. This technique will facilitate experiments that require highly purified preparations of PGCs including cell culture and gene expression analyses. (C) 1996 Academic Press, Inc.

引用

页码：468 / 472

页数：5

共 18 条

[1] RAPID ISOLATION OF DESIRED SEQUENCES FROM LONE LINKER PCR AMPLIFIED CDNA MIXTURES - APPLICATION TO IDENTIFICATION AND RECOVERY OF EXPRESSED SEQUENCES IN CLONED GENOMIC DNA [J].