Multichromosomal genome structure and confirmation of diazotrophy in novel plant-associated Burkholderia species

被引：55

作者：

Martinez-Aguilar, Lourdes ^{[1
]}

Diaz, Rafael ^{[1
]}

Pena-Cabriales, Juan Jose ^{[2
]}

Estrada-de los Santos, Paulina ^{[1
]}

Dunn, Michael F. ^{[1
]}

Caballero-Mellado, Jesus ^{[1
]}

机构：

[1] Univ Nacl Autonoma Mexico, Ctr Ciencias Genom, Cuernavaca 62191, Morelos, Mexico

[2] Ctr Invest & Estudios Avanzados, Guanajuato, Mexico

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2008年 / 74卷 / 14期

关键词：

D O I：

10.1128/AEM.00201-08

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Pulsed-field gel electrophoresis and 16S rRNA hybridization experiments showed that multichromosome genome structures and very large genome sizes (6.46 to 8.73 Mb) are prevalent in novel plant-associated Burkholderia species. N-15(2) isotope dilution assays revealed unambiguous diazotrophy in these novel species. nifH gene sequence analysis, often used to determine phylogenetic relatedness among diazotrophs, showed tight clusters of Burkholderia species, which were clearly distinct from those of other diazotrophs.

引用

页码：4574 / 4579

页数：6

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