Electrochromatographic enantioseparation using chiral ligand exchange monolithic sol-gel column

被引：53

作者：

Chen, Z ^{[1
]}

Nishiyama, T ^{[1
]}

Uchiyama, K ^{[1
]}

Hobo, T ^{[1
]}

机构：

[1] Tokyo Metropolitan Univ, Grad Sch Engn, Dept Appl Chem, Tokyo 1920397, Japan

来源：

ANALYTICA CHIMICA ACTA | 2004年 / 501卷 / 01期

关键词：

chiral separation; monolithic column; capillary electrochromatography; ligand exchange; chiral stationary phase; micro-liquid chromatography;

D O I：

10.1016/j.aca.2003.09.012

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This paper describes the development of a monolithic sol-gel column modified with L-hydroxyproline as a ligand exchange chiral stationary phase. It has been demonstrated that the monolithic chiral stationary phase can be used for the enantioseparation of dansyl amino acids, free amino acids, hydroxy acids, and dipeptides by capillary electrochromatography and micro-liquid chromatography. The recommended mobile phase was acetonitrile/0.50mM Cu(Ac)(2)-50 mM NH4Ac (7:3) adjusted to pH 6.5. The characteristics of the monolithic column using hydroxyproline as chiral selector in CEC have been discussed. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：17 / 23

页数：7

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