Cloning and heterologous expression of hematin-dependent catalase produced by Lactobacillus plantarum CNRZ 1228

被引:43
作者
Abriouel, H
Herrmann, A
Stärke, J
Yousif, NMK
Wijaya, A
Tauscher, B
Holzapfel, W
Franz, CMAP
机构
[1] Fed Res Ctr Nutr, Inst Biotechnol & Mol Biol, D-76131 Karlsruhe, Germany
[2] Fed Res Ctr Nutr, Inst Biol Chem, D-76131 Karlsruhe, Germany
关键词
D O I
10.1128/AEM.70.1.603-606.2004
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Lactobacillus plantarum CNRZ 1228 exhibited heme-dependent catalase activity under environmental conditions similar to those encountered during sausage fermentation. The 1,455-bp catalase gene (katL) was cloned and encoded a protein of 484 amino acids. Expression of katL in a heterologous host showed that katL encodes a functional catalase. PCR screening of selected strains of lactic acid bacteria for katL indicated the presence of similar genes in other strains of lactobacilli.
引用
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页码:603 / 606
页数:4
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