Feeding of nutrients delays apoptotic death in fed-batch cultures of recombinant NS0 myeloma cells

被引：25

作者：

DiStefano, DJ

Mark, GE

Robinson, DK

机构：

[1] Department of Cellular and Molecular Biology, Merck Research Laboratories, Mail Stop R80Y-160, Rahway, NJ 07065

[2] Schering-Plough Research Institute, Union, NJ 07083

来源：

BIOTECHNOLOGY LETTERS | 1996年 / 18卷 / 09期

关键词：

D O I：

10.1007/BF00129733

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The addition of concentrated nutrient solutions in fed-batch cultures of recombinant GS-NSO cells delays the onset of apoptosis by 24 to 48 hours and decreases the specific rate of apoptotic death approximately two-fold. This decrease in apoptosis appears primarily responsible for the increase in culture longevity that results in five- to ten-fold increases in final product concentrations in fed-batch cultures.

引用

页码：1067 / 1072

页数：6

共 13 条

[1]

[Anonymous], [No title captured]

[2] HIGH-LEVEL EXPRESSION OF A RECOMBINANT ANTIBODY FROM MYELOMA CELLS USING A GLUTAMINE-SYNTHETASE GENE AS AN AMPLIFIABLE SELECTABLE MARKER [J].

BEBBINGTON, CR ;

RENNER, G ;

THOMSON, S ;

KING, D ;

ABRAMS, D ;

YARRANTON, GT .

BIO-TECHNOLOGY, 1992, 10 (02) :169-175

[3]

BIBILA T, 1994, ANN NY ACAD SCI, V745, P277

[4] IN PURSUIT OF THE OPTIMAL FED-BATCH PROCESS FOR MONOCLONAL-ANTIBODY PRODUCTION [J].

BIBILA, TA ;

ROBINSON, DK .

BIOTECHNOLOGY PROGRESS, 1995, 11 (01) :1-13

[5] STARVATION-INDUCED PROGRAMMED DEATH OF HYBRIDOMA CELLS, PREVENTION BY AMINO-ACID MIXTURES [J].

FRANEK, F .

BIOTECHNOLOGY AND BIOENGINEERING, 1995, 45 (01) :86-90

[6] MATHEMATICAL DESCRIPTIONS OF HYBRIDOMA CULTURE KINETICS .3. SIMULATION OF FED-BATCH BIOREACTORS [J].

GLACKEN, MW ;

HUANG, C ;

SINSKEY, AJ .

JOURNAL OF BIOTECHNOLOGY, 1989, 10 (01) :39-65

[7]

GOULD SL, 1993, C CELL TISS CULT SAN

[8] DEVELOPMENT OF A SERUM-FREE MEDIUM FOR GROWTH OF NS-1 MOUSE MYELOMA CELLS AND ITS APPLICATION TO THE ISOLATION OF NS-1 HYBRIDOMAS [J].

KAWAMOTO, T ;

SATO, JD ;

LE, A ;

MCCLURE, DB ;

SATO, GH .

ANALYTICAL BIOCHEMISTRY, 1983, 130 (02) :445-453

[9] SPONTANEOUS APOPTOSIS IN NS-1 MYELOMA CULTURES - EFFECTS OF CELL-DENSITY, CONDITIONED MEDIUM AND ACID PH [J].

KLUCK, RM ;

CHAPMAN, DE ;

EGAN, M ;

MCDOUGALL, CA ;

HARMON, BV ;

MOSS, DJ ;

KERR, JFR ;

HALLIDAY, JW .

IMMUNOBIOLOGY, 1993, 188 (1-2) :124-133

[10] STRATEGIES TO EXTEND LONGEVITY OF HYBRIDOMAS IN CULTURE AND PROMOTE YIELD OF MONOCLONAL-ANTIBODIES [J].

LUAN, YT ;

MUTHARASAN, R ;

MAGEE, WE .

BIOTECHNOLOGY LETTERS, 1987, 9 (10) :691-696

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