Purification and characterization of recombinant lipid storage protein-2 from Drosophila melanogaster

被引：16

作者：

Arrese, Estela L. ^{[1
]}

Rivera, Laticia ^{[1
]}

Hamada, Masakazu ^{[1
]}

Soulages, Jose L. ^{[1
]}

机构：

[1] Oklahoma State Univ, Dept Biochem & Mol Biol, Noble Res Ctr 146, Stillwater, OK 74078 USA

来源：

PROTEIN AND PEPTIDE LETTERS | 2008年 / 15卷 / 09期

基金：

美国国家卫生研究院;

关键词：

lipid storage droplet protein; adipocytes; fat body; Drosophila; lipolysis;

D O I：

10.2174/092986608785849191

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lipid storage protein 2 (Lsd 2) is a conserved insect protein that belongs to the small PAT family of proteins. PAT proteins are found associated to the lipid droplets of adipocytes and play significant roles in the regulation of triacylglycerides metabolism. Here we describe the expression and purification of Lsd2, its reconstitution in lipoprotein particles, the location of putative lipid binding sites and its secondary structure. This study provides the starting point for future studies on the mechanism of function of Lsd2. The similarities and differences between Lsd1 and Lsd2, the only PAT proteins found in insects, are discussed.

引用

页码：1027 / 1032

页数：6

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