Physical and functional interactions between the transcription factor PU.1 and the coactivator CBP

被引：104

作者：

Yamamoto, H ^{[1
]}

Kihara-Negishi, F ^{[1
]}

Yamada, T ^{[1
]}

Hashimoto, Y ^{[1
]}

Oikawa, T ^{[1
]}

机构：

[1] Sasaki Inst, Dept Cell Genet, Chiyoda Ku, Tokyo 1010062, Japan

来源：

ONCOGENE | 1999年 / 18卷 / 07期

关键词：

PU.1; CBP; transcription factor;

D O I：

10.1038/sj.onc.1202427

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Yeast two-hybrid system was employed to isolate novel proteins that physically interact with PU.1, a member of Ets family transcription factors. Sequence analyses of several isolated clones positive for beta-galactosidase activity revealed that one of these clones was confirmed to encode a transcriptional coactivator, CREB binding protein (CBP), GST binding assay showed that the interacting sites were located at the transcriptional activation domain of PU,1 through 74-122 and the region spanning residues 1283-1915 of CBP. CBP potentiated PU.1-mediated transcription of the reporter gene driven by the multimerized PU.1-binding sites, suggesting that CBP functions as a coactivator for PU.1. Considering that CBP is a limited cellular component to function as a coactivator for several transcription factors, CBP may mediate synergistic and antagonistic interactions between PU.1 and other transcription factors during the process of hematopoietic cell differentiation.

引用

页码：1495 / 1501

页数：7

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