A TALE of Two Nucleases: Gene Targeting for the Masses?

被引：42

作者：

Clark, Karl J. ^{[1
]}

Voytas, Daniel F. ^{[2
,3
]}

Ekker, Stephen C. ^{[1
]}

机构：

[1] Mayo Clin, Dept Biochem & Mol Biol, Mayo Addict Res Ctr, Rochester, MN 55905 USA

[2] Univ Minnesota, Dept Genet Cell Biol & Dev, Minneapolis, MN USA

[3] Univ Minnesota, Ctr Genom Engn, Minneapolis, MN USA

来源：

ZEBRAFISH | 2011年 / 8卷 / 03期

关键词：

DISRUPTION;

D O I：

10.1089/zeb.2011.9993

中图分类号：

Q [生物科学];

学科分类号：

090105 [作物生产系统与生态工程];

摘要：

Genome editing appears poised to enter an exciting new era. Targeted double-stranded breaks due to custom restriction enzymes are powerful nucleating events for the induction of local changes in the genome. The zinc finger nuclease (ZFN) platform established the potential of this approach for the zebrafish, but access to high quality reagents has been a major bottleneck for the field. However, two groups recently report successful somatic and germline gene modification using a new nuclease architecture, transcription activator-like effector nucleases (TALENs). TALEN construction is simpler, potentially more reliable, and in the few cases examined, shows fewer off-target effects than corresponding ZFNs. TALENs promise to bring gene targeting to the majority of zebrafish laboratories.

引用

页码：147 / 149

页数：3

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