Delivery of antisense oligonucleotides using cholesterol-modified sense dendrimers and cationic lipids

被引：21

作者：

Chaltin, P

Margineanu, A

Marchand, D

Van Aerschot, A

Rozenski, J

De Schryver, F

Herrmann, A

Müllen, K

Juliano, R

Fisher, MH

Kang, HM

De Feyter, S

Herdewijn, P

机构：

[1] Katholieke Univ Leuven, Rega Inst, Med Chem Lab, B-3000 Louvain, Belgium

[2] Katholieke Univ Leuven, Lab Photochem & Spect, B-3000 Louvain, Belgium

[3] Univ N Carolina, Sch Med, Dept Pharmacol, Chapel Hill, NC 27599 USA

[4] Max Planck Inst Polymer Res, D-55021 Mainz, Germany

来源：

BIOCONJUGATE CHEMISTRY | 2005年 / 16卷 / 04期

关键词：

D O I：

10.1021/bc050022g

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Cholesterol modified mono-, di-, and tetrameric oligonucleotides were synthesized and hybridized with antisense oligonucleotides to study their incorporation in cationic liposomes together with the influence of this dendrimeric delivery system on biological activity. Electrostatic interactions seem to play the most important role during complexation with cationic lipids. This oligonucleotide formulation gives a small but significant increase in the inhibition of P-glycoprotein expression in a cellular system.

引用

页码：827 / 836

页数：10

共 24 条

[1] Inhibition of certain strains of HIV-1 by cell surface polyanions in the form of cholesterol-labeled oligonucleotides [J].