Purification and properties of an endo-inulinase from an Arthrobacter sp.

被引：39

作者：

Kang, SI

Chang, YJ

Oh, SJ

Kim, SI ^{[1
]}

机构：

[1] Seoul Natl Univ, Coll Agr & Life Sci, Dept Agr Chem, Suwon 441744, South Korea

[2] Seoul Natl Univ, Coll Agr & Life Sci, New Biomat Agr Res Ctr, Suwon 441744, South Korea

来源：

BIOTECHNOLOGY LETTERS | 1998年 / 20卷 / 10期

关键词：

endo-inulinase; inulo-oligosaccharides; Arthrobacter so;

D O I：

10.1023/A:1005405510397

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Extracellular endo-inulinase of Arthrobacter sp. S37 was purified 63-fold, giving a single band on PAGE with activity staining. The M-r was estimated as 75 kDa by SDS-PAGE. The first 31 amino acids of the N-terminal sequence was determined. The endo-inulinase hydrolyzed inulin mainly into inulo-triose (F3), inulo-tetraose (F4) and inulo-pentaose (F5) optimally at pH 7.5 and 50 degrees C.

引用

页码：983 / 986

页数：4

共 11 条

[1] MOLECULAR AND KINETIC-PROPERTIES OF ASPERGILLUS-FICUUM INULINASES [J].