Self-assembled monolayer of sugar-carrying polymer chain: Sugar balls from 2-methacryloyloxyethyl D-glucopyranoside

被引:65
作者
Yoshizumi, A [1 ]
Kanayama, N [1 ]
Maehara, Y [1 ]
Ide, M [1 ]
Kitano, H [1 ]
机构
[1] Toyama Univ, Dept Chem & Biochem Engn, Toyama 9308555, Japan
关键词
D O I
10.1021/la980374u
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A sugar-carrying polymer chain with a disulfide group (DTPA-PMEGlc) was prepared by the coupling of poly(2-methacryloyloxyethyl D-glucopyranoside) (PMEGlc), which carried an amino group at its end, with 3,3'-dithiodipropionic acid di-p-nitrophenyl ester. The polymer obtained was incubated with colloidal silver, and a self-assembled monolayer (SAM) of the polymer chain (PMEGlc) was formed on the surface of the colloid as evidenced by surface-enhanced Raman spectroscopy, cyclic voltammetry, dynamic light scattering, and ellipsometry. The silver colloid was largely stabilized by the modification with PMEGlc chains due to the formation of a thick diffuse layer on the surface. The critical flocculation concentration (CFC) of the modified colloid was not detectable, which is a contrast to the presence of CFC for the starting silver colloid dispersion (0.1 M NaCl at 25 degrees C). The polymer-coated colloids obtained ("sugar balls") were aggregated when a solution of lectin (concanavalin A (Con A) from Canavalia ensiformis) was added to the dispersion, due to a specific binding of D-glucopyranoside residues on the colloid particles by a tetrameric lectin molecule. The association constant (K-asn) for glucose residues on the colloid with Con A (7.1 x 10(5) M-1) was much larger than those for the complexation of Con a with small molecular weight sugars such as alpha-methyl D-glucopyranoside (4.9 x 10(3) M-1) due to the so-called "cluster effect". Thermodynamic parameters for the binding of Con A to sugar residues in the SAM of PMEGlc clearly showed that the binding is governed by entropy change (Delta S degrees = 108 J/K.mol). The usability of polymeric SAM in the biomedical field was strongly suggested.
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页码:482 / 488
页数:7
相关论文
共 47 条
[1]  
AOI K, 1995, MACROMOLECULES, V28, P591
[2]   POLYMERIZATION OF GLYCOLIDE PROMOTED BY OMEGA-AL-ALKOXIDE POLY(EPSILON-CAPROLACTONE) MACROINITIATORS AND FORMATION OF STABLE COLLOIDAL DISPERSIONS [J].
BARAKAT, I ;
DUBOIS, P ;
JEROME, R ;
TEYSSIE, P ;
MAZUREK, M .
MACROMOLECULAR SYMPOSIA, 1994, 88 :227-244
[3]  
BOHN L, 1989, POLYM HDB, P459
[4]  
BOVEY FA, 1972, HIGH RESOLUTION NMR
[5]   SURFACE RAMAN-SCATTERING OF SELF-ASSEMBLED MONOLAYERS FORMED FROM 1-ALKANETHIOLS AT AG [J].
BRYANT, MA ;
PEMBERTON, JE .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (10) :3629-3637
[6]   SURFACE RAMAN-SCATTERING OF SELF-ASSEMBLED MONOLAYERS FORMED FROM 1-ALKANETHIOLS - BEHAVIOR OF FILMS AT AU AND COMPARISON TO FILMS AT AG [J].
BRYANT, MA ;
PEMBERTON, JE .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1991, 113 (22) :8284-8293
[7]   PREPARATION AND CHARACTERIZATION OF POLYSTYRENE LATEXES BEARING DISACCHARIDE SURFACE GROUPS [J].
CHARREYRE, MT ;
BOULLANGER, P ;
DELAIR, T ;
MANDRAND, B ;
PICHOT, C .
COLLOID AND POLYMER SCIENCE, 1993, 271 (07) :668-679
[8]  
Creighton TE, 1993, PROTEINS STRUCTURES
[9]   Formation of a self-assembled monolayer by adsorption of thiophene on Au(111) and its photooxidation [J].
Dishner, MH ;
Hemminger, JC ;
Feher, FJ .
LANGMUIR, 1996, 12 (26) :6176-6178
[10]   COLORIMETRIC METHOD FOR DETERMINATION OF SUGARS AND RELATED SUBSTANCES [J].
DUBOIS, M ;
GILLES, KA ;
HAMILTON, JK ;
REBERS, PA ;
SMITH, F .
ANALYTICAL CHEMISTRY, 1956, 28 (03) :350-356