Doublecortin expression in the adult rat telencephalon

被引:382
作者
Nacher, J [1 ]
Crespo, C
McEwen, BS
机构
[1] Rockefeller Univ, Neuroendocrinol Lab, New York, NY 10021 USA
[2] Univ Valencia, Dept Cell Biol, E-46100 Valencia, Spain
关键词
adult neurogenesis; immunocytochemistry; neuronal migration; PSA-NCAM;
D O I
10.1046/j.0953-816x.2001.01683.x
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Doublecortin (DCX) is a protein required for normal neuronal migration in the developing cerebral cortex, where it is widely expressed in both radially and tangentially migrating neuroblasts. Moreover, it has been observed in the adult rostral migratory stream, which contains the neuronal precursors traveling to the olfactory bulb. We have performed DCX immunocytochemistry in the adult rat brain to identify precisely the neuronal populations expressing this protein. Our observations confirm the presence of DCX immunoreactive cells with the characteristic morphology of migrating neuroblasts in the subventricular zone, rostral migratory stream and the main and accessory olfactory bulbs. We have also found putative migratory cells expressing DCX in regions were no adult neuronal migration has been described, as the corpus callosum, the piriform cortex layer III/endopiriform nucleus and the striatum. Surprisingly, many cells with the phenotype of differentiated neurons were DCX immunoreactive; e.g. certain granule neurons in the hilar border of the granular layer of the dentate gyrus, some neuronal types in the piriform cortex layer II, granule and periglomerular neurons in the main and accessory olfactory bulbs, and isolated cells in the striatum. Almost all DCX immunoreactive cells also express the polysialylated form of neural cell adhesion molecule and have a similar distribution to rat collapsin receptor-mediated protein-4, two molecules involved in neuronal structural plasticity. Given these results, we hypothesize that DCX expression in differentiated neurons could be related to its capacity for microtubule reorganization and that this fact could be linked to axonal outgrowth or synaptogenesis.
引用
收藏
页码:629 / 644
页数:16
相关论文
共 40 条
[1]  
Alonso G, 1997, J COMP NEUROL, V384, P181
[2]   TRANSPLANTATION OF CULTURED TYPE-1 ASTROCYTE CELL-SUSPENSIONS INTO YOUNG, ADULT AND AGED RAT CORTEX - CELL-MIGRATION AND SURVIVAL [J].
ANDERSSON, C ;
TYTELL, M ;
BRUNSOBECHTOLD, J .
INTERNATIONAL JOURNAL OF DEVELOPMENTAL NEUROSCIENCE, 1993, 11 (05) :555-568
[3]  
BAYER SA, 1982, SCIENCE, V216, P890, DOI 10.1126/science.7079742
[4]  
BENOWITZ LI, 1988, J NEUROSCI, V8, P339
[5]   X-linked female band heterotopia-male lissencephaly syndrome [J].
Berg, MJ ;
Schifitto, G ;
Powers, JM ;
Martinez-Capolino, C ;
Fong, CT ;
Myers, GJ ;
Epstein, LG ;
Walsh, CA .
NEUROLOGY, 1998, 50 (04) :1143-1146
[6]  
Bernier PJ, 1998, J NEUROSCI, V18, P2486
[7]  
Cajal S. R, 1955, STUDIES CEREBRAL COR
[8]   DIFFERENTIATION OF NEWLY BORN NEURONS AND GLIA IN THE DENTATE GYRUS OF THE ADULT-RAT [J].
CAMERON, HA ;
WOOLLEY, CS ;
MCEWEN, BS ;
GOULD, E .
NEUROSCIENCE, 1993, 56 (02) :337-344
[9]   In vivo induction of massive proliferation, directed migration, and differentiation of neural cells in the adult mammalian brain [J].
Fallon, J ;
Reid, S ;
Kinyamu, R ;
Opole, I ;
Opole, R ;
Baratta, J ;
Korc, M ;
Endo, TL ;
Duong, A ;
Nguyen, G ;
Karkehabadhi, M ;
Twardzik, D ;
Loughlin, S .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (26) :14686-14691
[10]   Doublecortin is a developmentally regulated, microtubule-associated protein expressed in migrating and differentiating neurons [J].
Francis, F ;
Koulakoff, A ;
Boucher, D ;
Chafey, P ;
Schaar, B ;
Vinet, MC ;
Friocourt, G ;
McDonnell, N ;
Reiner, O ;
Kahn, A ;
McConnell, SK ;
Berwald-Netter, Y ;
Denoulet, P ;
Chelly, J .
NEURON, 1999, 23 (02) :247-256