A modified Coomassie Brilliant Blue staining method at nanogram sensitivity compatible with proteomic analysis

被引：107

作者：

Wang, Xuchu ^{[1
]}

Li, Xiaofang ^{[1
]}

Li, Yinxin ^{[1
]}

机构：

[1] Chinese Acad Sci, Inst Bot, Key Lab Photsynth & Environm Mol Physiol, Beijing 100093, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2007年 / 29卷 / 10期

关键词：

Coomassie Brilliant Blue; mass spectrometry; plant proteomics; protein staining method; two-dimensional electrophoresis;

D O I：

10.1007/s10529-007-9425-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A more sensitive and convenient Coomassie Brilliant Blue (CBB) staining method for visualizing proteins was developed. Compared with the modifications include the supplement of 10% (v/v) methanol into the fixing solution, an increase of an additional sensitization step and CBB raised from 0.1 to 0.125%. The improved method can detect proteins at nanogram level. The improved method is more sensitive than Blue Silver and more convenient than the Silver protocol. Mass spectrometry results confirmed that it is suitable for subsequent proteomic research.

引用

页码：1599 / 1603

页数：5

共 13 条

[1] Blue silver: A very sensitive colloidal Coomassie G-250 staining for proteome analysis [J].