Molecular mechanism of MexR-mediated regulation of MexAB-OprM efflux pump expression in Pseudomonas aeruginosa

被引：23

作者：

Saito, K ^{[1
]}

Eda, S ^{[1
]}

Maseda, H ^{[1
]}

Nakae, T ^{[1
]}

机构：

[1] Tokai Univ, Sch Med, Dept Mol Life Sci, Kanagawa 2591193, Japan

来源：

FEMS MICROBIOLOGY LETTERS | 2001年 / 195卷 / 01期

关键词：

efflux pump; antibiotic resistance; repressor; multidrug; gene expression; regulation;

D O I：

10.1111/j.1574-6968.2001.tb10492.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

To elucidate the molecular mechanism by which the MexR repressor regulates expression of the MexAB-OprM efflux pump, we investigated MexR and the mexR-mexA intergenic DNA (mexOP) interaction. and transcription of the mexA-lacZ reporter gene containing different lengths or mexOP. Homogeneously purified MexR bound specifically to mexOP proximal to mexR. The mexOP-lacZ fusion gene lacking the region immediately proximal to mexR showed minimum enzyme activity, thereby suggesting that a promoter element is located between mexR and the MexR-binding sires. These observations explain the mechanism of self-regulation of mexR expression as well as low and elevated expression of MexAB-OprM in the wild-type strain and nalB-type mutant, respectively, of Pseudomonas aeruginosa. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：23 / 28

页数：6

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