Caldendrin, a novel neuronal calcium-binding protein confined to the somato-dendritic compartment

被引:84
作者
Seidenbecher, CI
Langnaese, K
Sanmartí-Vila, L
Boeckers, TM
Smalla, KH
Sabel, BA
Garner, CC
Gundelfinger, ED
Kreutz, MR
机构
[1] Otto Von Guericke Univ, Inst Med Psychol, AG Mol & Cellular Neurobiol, D-39120 Magdeburg, Germany
[2] Leibniz Inst Neurobiol, Dept Neurochem & Mol Biol, D-39118 Magdeburg, Germany
[3] Westfalische Wilhelms Universitat, Inst Anat, AG Mol Neuroendocrinol, D-48149 Munster, Germany
[4] Otto Von Guericke Univ, Inst Pharmacol & Toxicol, D-39120 Magdeburg, Germany
[5] Univ Alabama, Dept Neurobiol, Birmingham, AL 35294 USA
关键词
D O I
10.1074/jbc.273.33.21324
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Using antibodies against synaptic protein preparations, we cloned the cDNA of a new Ca2+-binding protein. Its C-terminal portion displays significant similarity with calmodulin and contains two EF-hand motifs. The corresponding mRNA is highly expressed in rat brain, primarily in cerebral cortex, hippocampus, and cerebellum; its expression appears to be restricted to neurons. Transcript levels increase during postnatal development. A recombinant C-terminal protein fragment binds Ca2+ as indicated by a Ca2+-induced mobility shift in SDS-polyacrylamide gel electrophoresis. Antisera generated against the bacterial fusion protein recognize a brain-specific protein doublet with apparent molecular masses of 33 and 36 kDa, These data are confirmed by in vitro translation, which generates a single 36-kDa polypeptide, and by the heterologous expression in 293 cells, which yields a 33/36-kDa doublet comparable to that found in brain. On two-dimensional gels, the 33-kDa band separates into a chain of spots plausibly due to differential phosphorylation. This view is supported by in situ phosphorylation studies in hippocampal slices. Most of the immunoreactivity is detectable in cytoskeletal preparations with a further enrichment in the synapse-associated cytomatrix. These biochemical data, together with the ultra-structural localization in dendrites and the postsynaptic density, strongly suggest an association with the somato-dendritic cytoskeleton, Therefore, this novel Ca2+-binding protein was named caldendrin.
引用
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页码:21324 / 21331
页数:8
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