Measurement of the 13C/12C ratio of soil-plant individual sugars by gas chromatography/combustion/isotope-ratio mass spectrometry of silylated derivatives

被引:31
作者
Derrien, D [1 ]
Balesdent, J [1 ]
Marol, C [1 ]
Santaella, C [1 ]
机构
[1] CNRS, CEA DSV DEVM, LEMIR, F-13108 St Paul Les Durance, France
关键词
D O I
10.1002/rcm.1269
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Carbohydrate is an important pool in the terrestrial carbon cycle. The potential offered by natural and artificial C-13-labelling techniques should therefore be applied to the investigation of the dynamics of individual sugars in soils. For this reason, we evaluated the method of C-13 sugar analysis by gas chromatography/combustion/isotope-ratio mass spectrometry (GC/C/IRMS) after hydrolysis and direct trimethylsilylation. Trimethylsilylation involved the addition of several carbon atoms per sugar. These atoms have to be taken into account in the estimation of the carbon isotope ratio. The analysis of standard and natural pentoses and hexoses of known C-13 enrichments revealed that the number of analysed added carbon atoms was less than expected from stoichiometry. This was attributed to incomplete derivatization and/or incomplete oxidation of methylsilyl carbon before IRMS. Using a calibration of the number of analysed added carbon atoms, the isotope excess of enriched samples could be determined with a relative error close to 5%. Concerning the determination of natural abundances by GC/C/IRMS, we could measure the delta 13C of standard C3- and C4-derived sugars with an accuracy of +/-1.5parts per thousand using the previous calibration. We were able to apply this technique to plant-soil systems labelled by pulse-chase of (CO2)-C-13, revealing the nature and dynamics of sugars in the plant rhizosphere. Copyright (C) 2003 John Wiley Sons, Ltd.
引用
收藏
页码:2626 / 2631
页数:6
相关论文
共 18 条
[1]   Simultaneous determination by capillary gas chromatography of organic acids, sugars, and sugar alcohols in plant tissue extracts as their trimethylsilyl derivatives [J].
Adams, MA ;
Chen, ZL ;
Landman, P ;
Colmer, TD .
ANALYTICAL BIOCHEMISTRY, 1999, 266 (01) :77-84
[2]   Determination of neutral and acidic sugars in soil by capillary gas-liquid chromatography after trifluoroacetic acid hydrolysis [J].
Amelung, W ;
Cheshire, MV ;
Guggenberger, G .
SOIL BIOLOGY & BIOCHEMISTRY, 1996, 28 (12) :1631-1639
[3]  
Balesdent J., 1996, Mass spectrometry of soils., P83
[4]  
Chaplin M. F., 1994, CARBOHYDRATE ANAL PR, P27
[5]   Practical and theoretical considerations in the gas chromatography/combustion/isotope ratio mass spectrometry δ13C analysis of small polyfunctional compounds [J].
Docherty, G ;
Jones, V ;
Evershed, RP .
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2001, 15 (09) :730-738
[6]   COMPOSITION AND DYNAMICS OF DISSOLVED CARBOHYDRATES AND LIGNIN-DEGRADATION PRODUCTS IN 2 CONIFEROUS FORESTS, NE BAVARIA, GERMANY [J].
GUGGENBERGER, G ;
ZECH, W .
SOIL BIOLOGY & BIOCHEMISTRY, 1994, 26 (01) :19-27
[7]   Comparative organic geochemistries of soils and marine sediments [J].
Hedges, JI ;
Oades, JM .
ORGANIC GEOCHEMISTRY, 1997, 27 (7-8) :319-361
[8]   Simultaneous determination of sugars, sugar alcohols, acids and amino acids in apricots by gas chromatography-mass spectrometry [J].
Katona, ZF ;
Sass, P ;
Molnár-Perl, I .
JOURNAL OF CHROMATOGRAPHY A, 1999, 847 (1-2) :91-102
[9]   Determination of carbohydrates in two ferrallitic soils: Analysis by capillary gas chromatography after derivatization by silylation [J].
LarreLarrouy, MC ;
Feller, C .
SOIL BIOLOGY & BIOCHEMISTRY, 1997, 29 (9-10) :1585-1589
[10]   Stable isotopic analysis of individual carbohydrates by gas chromatographic combustion/isotope ratio mass spectrometry [J].
Macko, SA ;
Ryan, M ;
Engel, MH .
CHEMICAL GEOLOGY, 1998, 152 (1-2) :205-210