Protein separation via polyelectrolyte coacervation: Selectivity and efficiency

被引:155
作者
Wang, YF [1 ]
Gao, JY [1 ]
Dubin, PL [1 ]
机构
[1] INDIANA UNIV PURDUE UNIV,DEPT CHEM,INDIANAPOLIS,IN 46202
关键词
D O I
10.1021/bp960013+
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Selective phase separation with polyelectrolytes can be used to separate a mixture of proteins. The efficiency of separation was examined using the cationic polyelectrolyte poly(diallyldimethylammonium chloride) and the model proteins bovine serum albumin, beta-lactoglobulin, gamma-globulin, and ribonuclease A. The coacervation yield for individual proteins and the degree of separation, for selected protein pairs, were studied as a function of polymer molecular weight, ionic strength, and pH.
引用
收藏
页码:356 / 362
页数:7
相关论文
共 23 条
[1]  
AHMED LS, 1994, PURE APPL CHEM A, V31, P17, DOI 10.1080/10601329409349714
[2]  
[Anonymous], 1990, INTRO DYNAMIC LIGHT
[3]  
Burgess D. J., 1994, MACROMOLECULAR COMPL, P285
[4]  
BURGESS DJ, 1984, J COLLOID INTERF SCI, V98, P1
[5]   POLYMER DOSAGE CONSIDERATIONS IN POLY-ELECTROLYTE PRECIPITATION OF PROTEIN [J].
CLARK, KM ;
GLATZ, CE .
BIOTECHNOLOGY PROGRESS, 1987, 3 (04) :241-247
[6]  
DEJONG HGB, 1949, COLLOID SCI, V2, pCH10
[7]   PROTEIN-PURIFICATION BY SELECTIVE PHASE-SEPARATION WITH POLYELECTROLYTES [J].
DUBIN, PL ;
GAO, J ;
MATTISON, K .
SEPARATION AND PURIFICATION METHODS, 1994, 23 (01) :1-16
[8]  
IZUMI T, 1994, PURE APPL CHEM, V31, P31
[9]   STOICHIOMETRIC COMPLEXATION OF HUMAN-SERUM ALBUMIN WITH STRONGLY ACIDIC AND BASIC POLY-ELECTROLYTES [J].
KOKUFUTA, E ;
SHIMIZU, H ;
NAKAMURA, I .
MACROMOLECULES, 1982, 15 (06) :1618-1621
[10]  
Ladisch M. R., 1990, PROTEIN PURIFICATION