Protein separation via polyelectrolyte coacervation: Selectivity and efficiency

被引：155

作者：

Wang, YF ^{[1
]}

Gao, JY ^{[1
]}

Dubin, PL ^{[1
]}

机构：

[1] INDIANA UNIV PURDUE UNIV,DEPT CHEM,INDIANAPOLIS,IN 46202

来源：

BIOTECHNOLOGY PROGRESS | 1996年 / 12卷 / 03期

关键词：

D O I：

10.1021/bp960013+

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Selective phase separation with polyelectrolytes can be used to separate a mixture of proteins. The efficiency of separation was examined using the cationic polyelectrolyte poly(diallyldimethylammonium chloride) and the model proteins bovine serum albumin, beta-lactoglobulin, gamma-globulin, and ribonuclease A. The coacervation yield for individual proteins and the degree of separation, for selected protein pairs, were studied as a function of polymer molecular weight, ionic strength, and pH.

引用

页码：356 / 362

页数：7

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