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Overexpression of plant uncoupling mitochondrial protein in transgenic tobacco increases tolerance to oxidative stress

被引：62
作者：
Brandalise, M
Maia, IG
Borecky, J
Vercesi, AE
Arruda, P
机构：
[1] Univ Estadual Campinas, Ctr Biol Mol & Engn Genet, BR-13083970 Campinas, SP, Brazil
[2] Univ Estadual Paulista, Dept Genet, IB, Botucatu, SP, Brazil
[3] Univ Estadual Campinas, Dept Patol Clin NMCE, FCM, Campinas, SP, Brazil
[4] Univ Estadual Campinas, Dept Genet & Evolucao, IB, Campinas, SP, Brazil
来源：
JOURNAL OF BIOENERGETICS AND BIOMEMBRANES | 2003年 / 35卷 / 03期
基金：
巴西圣保罗研究基金会;
关键词：
transgenic tobacco; plant uncoupling protein; oxidative stress; constitutive overexpression;
D O I：
10.1023/A:1024603530043
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
An Arabidopsis thaliana cDNA clone encoding a plant uncoupling mitochondrial protein (AtPUMP1) was overexpressed in transgenic tobacco plants. Analysis of the AtPUMP1 mRNA content in the transgenic lines, determined by Northern blot, revealed variable levels of transgene expression. Antibody probing of Western blots of mitochondrial proteins from three independent transgenic lines showed significant accumulation of AtPUMP1 in this organelle. Overproduction of AtPUMP1 in transgenic tobacco plants led to a significant increase in tolerance to oxidative stress promoted by exogenous hydrogen peroxide as compared to wild-type control plants. These results provide the first biological evidence for a role of PUMP in protection of plant cells against oxidative stress damage.
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收藏
页码：203 / 209
页数：7
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