An air-molding technique for fabricating PDMS planar patch-clamp electrodes

被引：59

作者：

Klemic, KG

Klemic, JF

Sigworth, FJ ^{[1
]}

机构：

[1] Yale Univ, Dept Cellular & Mol Physiol, New Haven, CT 06520 USA

[2] Yale Univ, Dept Elect Engn & Appl Phys, New Haven, CT 06520 USA

来源：

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY | 2005年 / 449卷 / 06期

关键词：

ion channel; microfabrication; micromolding; PDMS;

D O I：

10.1007/s00424-004-1360-8

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

We present a new technique for fabricating planar patch electrodes in the laboratory. Planar electrodes are micromolded using a micron-sized stream of air to define an aperture in the silicone elastomer, polydimethylsiloxane (PDMS). We have previously demonstrated that planar PDMS electrodes make excellent patch electrodes after surface modification. We demonstrate single-channel measurements of the rSlo channel in Xenopus oocytes and whole-cell measurements in CHO and RBL mammalian cell lines, using planar PDMS electrodes.

引用

收藏

页码：564 / 572

页数：9

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