Evaluation of the growth inhibition strength of hydrocarbon solvents against Escherichia coli and Pseudomonas putida grown in a two-liquid phase culture system consisting of a medium and organic solvent

被引：18

作者：

Aono, R ^{[1
]}

Tsukagoshi, N ^{[1
]}

Miyamoto, T ^{[1
]}

机构：

[1] Tokyo Inst Technol, Grad Sch Biosci & Biotechnol, Dept Biol Informat, Midori Ku, Yokohama, Kanagawa 2268501, Japan

来源：

EXTREMOPHILES | 2001年 / 5卷 / 01期

关键词：

Escherichia coli; Pseudomonas putida; two-liquid phase culture system; growth inhibition strength; organic solvent; solvent toxicity; solvent resistance;

D O I：

10.1007/s007920000167

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The growth of microorganisms is often inhibited in a two-liquid phase culture system consisting of an aqueous medium and a large volume of hydrophobic solvent. Escherichia coli and Pseudomonas putida were cultured in a two-phase system containing a solvent with a log P-ow value in a range of 2.1 to 6.0. The increase in the cell mass was monitored by increase in turbidity of the medium phase. We devised a semiquantitative method to evaluate the growth inhibition strength of solvents based on the relative amount of bacterial growth occurring in the two-phase system. Analyses of growth of the bacteria by this method showed that the growth inhibition strength of a given solvent was usually but not always correlated inversely with its polarity It is clear that growth inhibition strength is not determined simply by polarity of the solvent.

引用

页码：11 / 15

页数：5

共 17 条

[1] EFFECTS OF ORGANIC-SOLVENTS ON GROWTH OF ESCHERICHIA-COLI K-12 [J].